A chromogenic alkaline phosphatase substrate
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4-Nitrophenyl Phosphate (sodium salt hydrate)

Item No. 34081

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
phosphoric acid, mono(4-nitrophenyl) ester, disodium salt, hexahydrate
CAS Number
333338-18-4
Synonyms
  • p-Nitrophenyl Phosphate
  • para-Nitrophenyl Phosphate
  • 4-NPP
  • pNPP
Molecular Formula
C6H4NO6P • 2Na [6H2O]
Formula Weight
371.1
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Ethanol: 10 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
222, 306 nm
SMILES
O=P([O-])([O-])OC(C=C1)=CC=C1[N+]([O-])=O.[Na+].[Na+].O.O.O.O.O.O
InChi Code
InChI=1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChi Key
KAKKHKRHCKCAGH-UHFFFAOYSA-L
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 g

    $54

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    10 g

    $102

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    25 g

    $158

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    50 g

    $290

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    Product Description

    4-Nitrophenyl phosphate (pNPP) is a chromogenic substrate for alkaline phosphatase.1 Upon hydrolysis by alkaline phosphatase, 4-nitrophenol is released, which can be quantified by colorimetric detection at 405 nm as a measure of alkaline phosphatase activity. It has been commonly used in ELISAs.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lorenz, U. Protein tyrosine phosphatase assays. Curr. Protoc. Immunol. 93, 11.17.11-11.17.12 (2011).