An amine-reactive fluorescent probe
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Fluorescamine

Item No. 34286

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-phenyl-spiro[furan-2(3H),1′(3′H)-isobenzofuran]-3,3′-dione
CAS Number
38183-12-9
Synonyms
  • Fluram
  • Ro 20-7234
Molecular Formula
C17H10O4
Formula Weight
278.3
Purity
≥95%
Emission
488 nm, in acetone
Excitation
370 nm, in acetone
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlDMSO:PBS (pH 7.2) (1:4): 0.2 mg/mlEthanol: 1 mg/ml
λmax
222, 237 nm
SMILES
O=C1OC2(C3=C1C=CC=C3)C(C(C4=CC=CC=C4)=CO2)=O
InChi Code
InChI=1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
InChi Key
ZFKJVJIDPQDDFY-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    25 mg

    $36

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    50 mg

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    100 mg

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    500 mg

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    Product Description

    Fluorescamine is an amine-reactive fluorescent probe.1 It has commonly been used as a reagent in fluorometric protein assays.2,3 It displays excitation/emission maxima of 370/488 nm, respectively, in acetone.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Chen, R.F., and Scott, C.H. Atlas of fluorescence spectra and lifetimes of dyes attached to protein. Anal. Lett. 18(4), 393-421 (1985).

    2. Udenfriend, S., Stein, S., Böhlen, P., et al. Fluorescamine: A reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science 178(4063), 871-872 (1972).

    3. Tsukagoshi, K., Tanaka, A., Nakajima, R., et al. On-line capillary zone electrophoretic separation-chemiluminescence detection of protein labeled with fluorescamine. Anal. Sci. 12, 525-528 (1996).