A colorimetric substrate for alkaline phosphatase
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BCIP (p-toluidine salt)

Item No. 34423

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-bromo-4-chloro-1H-indol-3-ol 3-(dihydrogen phosphate) compd. with 4-methylbenzenamine
CAS Number
6578-06-9
Synonyms
  • 5-Bromo-4-chloro-3-indoyl phosphate
Molecular Formula
C8H6BrClNO4P • C7H9N
Formula Weight
433.6
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlDMSO:PBS (pH 7.2) (1:4): 0.2 mg/ml
λmax
232 nm
SMILES
O=P(O)(OC1=CNC2=C1C(Cl)=C(Br)C=C2)O.NC3=CC=C(C)C=C3
InChi Code
InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChi Key
QEIFSLUFHRCVQL-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    100 mg

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    500 mg

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    Product Description

    BCIP is a colorimetric substrate for alkaline phosphatase.1,2 Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry (IHC) or Western blot (WB).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Epstein, E., Wolf, P.L., Horwitz, J.P., et al. An indigogenic reaction for alkaline phosphatase in disk electrophoresis. Am. J. Clin. Pathol. 48(5), 530-534 (1967).

    2. Wolf, P.L., Horwitz, J.P., Vasquez, J., et al. The indigogenic reaction for histochemical demonstration of alkaline and acid phosphatase. Experientia 23(3), 183-185 (1967).