A colorimetric α-amylase substrate
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2-Chloro-4-nitrophenyl α-D-maltotrioside

Item No. 34459

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-chloro-4-nitrophenyl O-α-D-glucopyranosyl-(1→4)-O-α-D-glucopyranosyl-(1→4)-α-D-glucopyranoside
CAS Number
118291-90-0
Synonyms
  • CNP-G3
Molecular Formula
C24H34ClNO18
Formula Weight
660.0
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: Slightly solublePBS (pH 7.2): 10 mg/ml
λmax
291 nm
SMILES
OC[C@@H](O[C@@H]([C@@H]([C@H]1O)O)OC2=C(C=C(C=C2)[N+]([O-])=O)Cl)[C@H]1O[C@H]3O[C@@H]([C@H]([C@@H]([C@H]3O)O)O[C@H]4O[C@@H]([C@H]([C@@H]([C@H]4O)O)O)CO)CO
InChi Code
InChI=1S/C24H34ClNO18/c25-8-3-7(26(37)38)1-2-9(8)39-22-18(35)15(32)20(11(5-28)41-22)44-24-19(36)16(33)21(12(6-29)42-24)43-23-17(34)14(31)13(30)10(4-27)40-23/h1-3,10-24,27-36H,4-6H2/t10-,11-,12-,13-,14+,15-,16-,17-,18-,19-,20-,21-,22+,23-,24-/m1/s1
InChi Key
KMYYNUOXSFGLNX-XFNLHOCBSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    100 mg

    $199

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    250 mg

    $398

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    500 mg

    $547

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    1 g

    $796

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    Product Description

    2-Chloro-4-nitrophenyl α-D-maltotrioside is a colorimetric substrate for α-amylases.1 Upon hydrolysis by α-amylases, 2-chloro-4-nitrophenol is released which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity. 2-Chloro-4-nitrophenyl α-D-maltotrioside has been used to characterize the activity of human pancreatic or salivary amylase.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Winn-Deen, E.S., David, H., Sigler, G., et al. Development of a direct assay for α-amylase. Clin. Chem. 34(10), 2005-2008 (1988).