Active • Host: Insect cells • AA: 2-170 • Tag: N-terminal His • MW: 21.4 kDa
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Calcineurin B (human, recombinant)

Item No. 34631

Technical Information
Synonyms
  • Calcineurin Subunit B Type 1
  • CALNB1
  • CNA2
  • CNB
  • CNB1
  • PPP3R1
  • Protein Phosphatase 2B Regulatory Subunit 1
  • Protein Phosphatase 3 Regulatory Subunit B α Isoform 1
Purity
≥87% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg determined by the LAL endotoxin assay
Source
Active recombinant human N-terminal His-tagged calcineurin B expressed in insect cells
Amino Acids
2-170
MW
21.4 kDa
Formulation (Request formulation change)
Lyophilized from sterile 50 mM Tris, pH 8.0, with 100 mM sodium chloride, 2 mM DTT and 10% glycerol
UniProt Accession №
P63098
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $422

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    Product Description

    Calcineurin B is the regulatory subunit of calcineurin, a serine/threonine phosphatase with roles in T cell activation, cardiac function, learning and memory, apoptosis, and cell cycle regulation.1,2 It is encoded by two genes in mammals, PPP3R1, which is expressed in all tissues except the testes, and the testicular-restricted isoform PP3R2.1,3 Calcineurin B is a calcium-binding protein that contains four EF-hand calcium-binding sites, and calcium binding to calcineurin B is essential to both the phosphatase activity of calcineurin A and binding of inhibitor complexes to calcineurin.4,5 Cayman's Calcineurin B (human, recombinant) protein can be used for binding assays. This protein consists of 187 amino acids and has a calculated molecular weight of 21.4 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Rusnak, F., and Mertz, P. Calcineurin: Form and function. Physiol. Rev. 80(4), 1483-1521 (2020).

    2. Mansuy, I. Calcineurin. xPharm: The Comprehensive Pharmacology Reference (2007).

    3. Wang, M.G., Yi, H., Guerini, D., et al. Calcineurin A alpha (PPP3CA), calcineurin A beta (PPP3CB) and calcineurin B (PPP3R1) are located on human chromosomes 4, 10q21→q22 and 2p16→p15 respectively. Cytogenet. Cell Genet. 72(2-3), 236-241 (1996).

    4. Stemmer, P.M., and Klee, C.B. Dual calcium ion regulation of calcineurin by calmodulin and calcineurin B. Biochemistry 33(22), 6859-6866 (1994).

    5. Feng, B., and Stemmer, P.M. Interactions of calcineurin A, calcineurin B, and Ca2+. Biochemistry 38(38), 12481-12489 (1999).