An amine-reactive fluorescent protein-labeling agent
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AMCA N-succinimidyl ester

Item No. 34668

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-amino-4-methyl-2-oxo-2H-1-benzopyran-3-acetic acid, 2,5-dioxo-1-pyrrolidinyl ester
CAS Number
113721-87-2
Synonyms
  • 7-Amino-4-methylcoumarin-3-acetic Acid N-succinimidyl ester
  • 7-Amino-4-methyl-3-coumarinylacetic Acid N-succinimidyl ester
Molecular Formula
C16H14N2O6
Formula Weight
330.3
Purity
≥90%
Emission
400-460 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlDMSO:PBS (pH 7.2) (1:3): 0.25 mg/ml
λmax
236, 359 nm
SMILES
O=C1OC2=C(C(C)=C1CC(ON3C(CCC3=O)=O)=O)C=CC(N)=C2
InChi Code
InChI=1S/C16H14N2O6/c1-8-10-3-2-9(17)6-12(10)23-16(22)11(8)7-15(21)24-18-13(19)4-5-14(18)20/h2-3,6H,4-5,7,17H2,1H3
InChi Key
ILKQVTIMOGNSAS-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

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    500 µg

    $36

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    1 mg

    $68

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    5 mg

    $288

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    10 mg

    $535

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    Product Description

    AMCA N-succinimidyl ester is an amine-reactive fluorescent probe and ester form of AMCA (Item No. 29184).1 It contains an N-hydroxysuccinimide ester that reacts with lysine residues to form photostable amide links. Upon activation with UV light, AMCA displays emission maxima of 400-460 nm. AMCA N-succinimidyl ester has commonly been used in multiplex immunophenotyping applications.2,3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Khalfan, H., Abuknesha, R., Rand-Weaver, M., et al. Aminomethyl coumarin acetic acid: A new fluorescent labelling agent for proteins. Histochem. J. 18(9), 497-499 (1986).

    2. Ferri, G.-L., Gaudio, R.M., Castello, I.F., et al. Quadruple immunofluorescence: A direct visualization method. J. Histochem. Cytochem. 45(2), 155-158 (1997).

    3. Nederlof, P.M., Robinson, D., Abuknesha, R., et al. Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences. Cytometry 10(1), 20-27 (1989).

    4. Gothot, A., Grosdent, J.-C., and Paulus, J.-M. A strategy for multiple immunophenotyping by image cytometry: Model studies using latex microbeads labeled with seven streptavidin-bound fluorochromes. Cytometry 24(3), 214-225 (1996).