An oxidant-sensitive fluorescent probe
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5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

Item No. 34692

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3′,6′-bis(acetyloxy)-2′,7′-dichloro-3-oxo-spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-ar-carboxylic acid
CAS Number
127770-45-0
Synonyms
  • CDFDA
Molecular Formula
C25H14Cl2O9
Formula Weight
529.3
Purity
≥85% (mixture of isomers)
Emission
530 nm
Excitation
504 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/ml
SMILES
O=C(O1)C2=C(C=CC(C(O)=O)=C2)C31C4=CC(Cl)=C(OC(C)=O)C=C4OC5=C3C=C(Cl)C(OC(C)=O)=C5.O=C(O6)C7=C(C=C(C(O)=O)C=C7)C86C9=CC(Cl)=C(OC(C)=O)C=C9OC%10=C8C=C(Cl)C(OC(C)=O)=C%10
InChi Code
InChI=1S/2C25H14Cl2O9/c1-10(28)33-21-8-19-15(6-17(21)26)25(14-4-3-12(23(30)31)5-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19;1-10(28)33-21-8-19-15(6-17(21)26)25(14-5-12(23(30)31)3-4-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19/h2*3-9H,1-2H3,(H,30,31)
InChi Key
LZMPJBOMBCYYMP-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    25 mg

    $63

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    50 mg

    $98

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    100 mg

    $134

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    Product Description

    5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1 Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2’,7’,-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation/emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hempel, S.L., Buettner, G.R., O'Malley, Y.Q., et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic. Biol. Med. 27(1-2), 146-159 (1999).