Human Thrombopoietin Receptor Reporter (TPOR) Assay System

INDIGO's Human Thrombopoietin Receptor (TPOR) reporter assay utilizes proprietary human cells that provide constitutive expression of the human thrombopoietin receptor.

JAK2 dependent phosphorylation and activation of the transcription factor STAT5 is a prominent outcome of TPOR activation, and it is the signaling pathway exploited by the reporter cells included in this assay kit. Specifically, INDIGO's Reporter Cells contain the luciferase reporter gene functionally linked to an engineered minimal promoter sequence with upstream tandem STAT5 genetic response element (GRE) sequences. Thrombopoietin activates TPOR in a dose-dependent manner, thereby triggering the JAK2/STAT signal transduction cascade. Activated STAT5 binds to its consensus GREs to nitiate the formation of a complete transcription complex that drives expression of the Luc reporter gene. Therefore, quantifying changes in luciferase activity from peptide-, drug- or antibody-treated reporter cells relative to that of ‘untreated’ cells provides a sensitive surrogate measure of changes in the activity of TPOR.

INDIGO’s Reporter Cells are transiently transfected and prepared as frozen stocks using a proprietary CryoMite^™ process. This cryo-preservation method allows for the immediate dispensing of healthy, division-competent reporter cells into assay plates. There is no need for intermediate treatment steps such as spin-and-rinse of cells, viability determinations or cell titer adjustments. [INDIGO Catalog Nos. IB20001, IB20002, IB20001-32]

WARNING This product is not for human or veterinary use.