A colorimetric α-glucosidase substrate
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4-Nitrophenyl α-D-Glucopyranoside

Item No. 35218

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-nitrophenyl-α-D-glucopyranoside
CAS Number
3767-28-0
Synonyms
  • p-Nitrophenyl α-D-Glucopyranoside
  • pNPG
  • para-Nitrophenyl-α-D-glucopyranoside
Molecular Formula
C12H15NO8
Formula Weight
301.2
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlDMSO: 10 mg/mlEthanol: Slightly solublePBS (pH 7.2): 0.3 mg/ml
λmax
221, 298 nm
SMILES
O[C@H]([C@H]([C@@H]([C@H](O1)CO)O)O)[C@H]1OC2=CC=C(C=C2)[N+]([O-])=O
InChi Code
InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11-,12+/m1/s1
InChi Key
IFBHRQDFSNCLOZ-ZIQFBCGOSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

    $38

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    5 g

    $179

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    10 g

    $334

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    Product Description

    4-Nitrophenyl α-D-glucopyranoside is a colorimetric substrate for α-glucosidases.1 Upon enzymatic cleavage, p-nitrophenol is released, which can be quantified by colorimetric detection at 405 nm as a measure of α-glucosidase activity. 4-Nitrophenyl α-D-glucopyranoside has also been used as a colorimetric substrate for glucansucrases.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zeng, L., Zhang, G., Liao, Y., et al. Inhibitory mechanism of morin on α-glucosidase and its anti-glycation properties. Food Funct. 7(9), 3953-3963 (2016).

    2. Binder, T.P., and Robyt, J.F. p-Nitrophenyl α-D-glucopyranoside, a new substrate for glucansucrases. Carbohydr. Res. 124(2), 287-299 (1983).