A chromogenic substrate for β-galactosidase
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5-bromo-3-indolyl-β-D-Galactopyranoside

Item No. 35299

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-bromo-1H-indol-3-yl β-D-galactopyranoside
CAS Number
97753-82-7
Synonyms
  • Bluo-Gal
Molecular Formula
C14H16BrNO6
Formula Weight
374.2
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 2 mg/mlEthanol: insolPBS (pH 7.2): insol
λmax
228 nm
SMILES
BrC1=CC=C2C(C(O[C@@H]3O[C@@H]([C@@H]([C@@H]([C@H]3O)O)O)CO)=CN2)=C1
InChi Code
InChI=1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
InChi Key
LINMATFDVHBYOS-MBJXGIAVSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 mg

    $32

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    500 mg

    $109

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    1 g

    $186

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    Product Description

    5-Bromo-3-indolyl-β-D-galactopyranoside (Bluo-Gal) is a cell-permeable chromogenic substrate for β-galactosidase.1 Upon enzymatic hydrolysis, it generates an indigo-colored precipitate that can be used to measure β-galactosidase activity. Bluo-Gal has been used to detect the expression of β-galactosidase in muscle from transgenic mice expressing E. coli lacZ, the gene that encodes β-galactosidase.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Gioglio, L., de Angelis, M.G.C., Boratto, R., et al. An improved method for β-galactosidase activity detection on muscle tissue. A light and electron microscopic study. Ann. Anat. 184(2), 153-157 (2002).