NF-κB (p105/p50) Rabbit Monoclonal Antibody (Clone RM299)

NF-κB is a ubiquitously expressed transcription factor composed of varied combinations of five subunits: p65/RelA, RelB, c-Rel, p105/p50, and p100/p52.^1,2,3 The NF-κB p50 subunit is a truncated version of p105 that lacks ankyrin repeat domains.² The p105 subunit can be phosphorylated by GSK3β, which prevents p105 processing and degradation in resting cells.³ In TNF-α-stimulated cells, GSK3β phosphorylation induces processing of p105 into p50, which forms a heterodimer with p65 and is translocated to the nucleus to initiate the transcription of a variety of genes involved in innate and adaptive immunity. The p105 subunit can also be phosphorylated by the IKKβ complex downstream of TNF receptor 1 (TNFR1), inducing processing of p105 into p50, or by tumor progression locus 2 (TPL2) downstream of toll-like receptor 4 (TLR4) activation by LPS. Increased protein levels of NF-κB, irrespective of cytoplasmic or nuclear localization, are associated with lower overall survival and disease-free survival in patients with solid tumors.⁴ Cayman’s NF-κB (p105/p50) Rabbit Monoclonal Antibody (Clone RM299) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Peterson, J.M., Bakkar, N., and Guttridge, D.C. NF-κB signaling in skeletal muscle health and disease. Current Topics in Developmental Biology 96, 85-119 (2011).

2. Chen, L.-F., and Greene, W.C. Shaping the nuclear action of NF-κB. Nat. Rev. Mol. Cell Biol. 5(5), 392-401 (2004).

3. Viatour, P., Merville, M.-P., Bours, V., et al. Phosphorylation of NF-κB and IκB proteins: Implications in cancer and inflammation. Trends Biochem. Sci. 30(1), 43-52 (2005).

4. Wu, D., Wu, P., Zhao, L., et al. NF-κB expression and outcomes in solid tumors: A systematic review and meta-analysis. Medicine (Baltimore) 94(40), e1687 (2015).