For immunochemical detection of Musashi-2
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Musashi-2 Rabbit Monoclonal Antibody (Clone RM422)

Item No. 35315

Product Insert (PDF)
Technical Information
Synonyms
  • MSI2
  • RNA-Binding Protein Musashi Homolog 2
Immunogen
Peptide from the internal region of Musashi-2
Clone Designation
RM422
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) Musashi-2
Species Reactivity
(+) Human(+) Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    Musashi-2 is an RNA-binding protein that regulates target mRNA stability and translation.1,2 It contains two RNA recognition motifs at its N-terminus that each contain two highly conserved core motifs, RNP-1 and RNP-2, and a C-terminal domain that is subject to alternative splicing to produce a short or long isoform of Musashi-2.3,4 Musashi-2 is ubiquitously expressed and localizes to the nucleus and cytoplasm.3,4,5 It is involved in the differentiation and self-renewal of stem cells, including embryonic, neural, and hematopoietic stem cells, as well as in the migration and proliferation of keratinocytes.1,2 Knockdown of MSI2, the gene encoding Mushashi-2, induces cell cycle arrest at the G1 phase and apoptosis in keratinocytes.2 Chromosomal translocations leading to the fusion of MSI2 with HOXA9, resulting in expression of Musashi-2-HOXA9 fusion proteins, are associated with chronic myeloid leukemia (CML).4 Cayman’s Musashi-2 Rabbit Monoclonal Antibody (Clone RM422) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sutherland, J.M., McLaughlin, E.A., Hime, G.R., et al. The Musashi family of RNA binding proteins: Master regulators of multiple stem cell populations. Transcriptional and translational regulation of stem cells 786, 233-245 (2013).

    2. Bennett, C.G., Riemondy, K., Chapnick, D.A., et al. Genome-wide analysis of Musashi-2 targets reveals novel functions in governing epithelial cell migration. Nucleic Acids Res. 44(8), 3788-3800 (2016).

    3. Sakakibara, S., Nakamura, Y., Satoh, H., et al. RNA-binding protein Musashi2: Developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS. J. Neurosci. 21(20), 8091-8107 (2001).

    4. Barbouti, A., Höglund, M., Johansson, B., et al. A novel gene, MSI2, encoding a putative RNA-binding protein is recurrently rearranged at disease progression of chronic myeloid leukemia and forms a fusion gene with HOXA9 as a result of the cryptic t(7;17)(p15;q23). Cancer Res. 63(6), 1202-1206 (2003).

    5. Sutherland, J.M., Sobinoff, A.P., Gunter, K.M., et al. Knockout of RNA binding protein MSI2 impairs follicle development in the mouse ovary: Characterization of MSI1 and MSI2 during folliculogenesis. Biomolecules 5(3), 1228-1244 (2015).