A fluorinated fluorescein dye
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Oregon Green™ 488

Item No. 35372

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2′,7′-difluoro-3′,6′-dihydroxy-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
CAS Number
195136-58-4
Synonyms
  • OG 488
  • 2’,7’-Difluorofluorescein
Molecular Formula
C20H10F2O5
Formula Weight
368.3
Purity
≥95%
Emission
514 nm
Excitation
490 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Chloroform: SolubleDMSO: Soluble
SMILES
O=C(O1)C2=CC=CC=C2C31C4=CC(F)=C(O)C=C4OC5=CC(O)=C(F)C=C53
InChi Code
InChI=1S/C20H10F2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChi Key
FJXJIUHGLVUXQP-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    25 mg

    $69

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    50 mg

    $115

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    100 mg

    $211

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    Product Description

    Oregon Green™ 488 is a fluorinated fluorescein dye.1,2 It exists as a tautomer in aqueous solutions.2 Oregon Green™ 488 has minimal pH dependence at physiological pH but it can be used as an indicator for intracellular pH due to its low pKa of 4.8.2,3 It is less susceptible to photobleaching than fluorescein. Oregon Green™ 488 has been conjugated to antibodies and proteins, as well as DNA, for use in labeling and detection.2,4,3 It displays excitation/emission maxima of 490/514 nm, respectively.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sun, W.-C., Gee, K.R., Klaubert, D.H., et al. Synthesis of fluorinated fluoresceins. The Journal of Organic Chemistry 62(19), 6469-6475 (1997).

    2. Orte, A., Crovetto, L., Talavera, E.M., et al. Absorption and emission study of 2',7'-difluorofluorescein and its excited-state buffer-mediated proton exchange reactions. J. Phys. Chem. A 109(5), 734-747 (2005).

    3. Leung, K., Chakraborty, K., Saminathan, A., et al. A DNA nanomachine chemically resolves lysosomes in live cells. Nat. Nanotechnol. 14(2), 176-183 (2019).

    4. Yu, P., Lasagna, M., Pawlyk, A.C., et al. IIAGlc inhibition of glycerol kinase: A communications network tunes protein motions at the allosteric site. Biochemistry 46(43), 12355-12365 (2007).