A fluorescent probe
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7-(Diethylamino)coumarin-3-carboxylic Acid

Item No. 35396

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-(diethylamino)-2-oxo-2H-1-benzopyran-3-carboxylic acid
CAS Number
50995-74-9
Synonyms
  • Coumarin D 1421
  • 7-DCCA
  • 7-Diethylamino-2-oxo-2H-chromen-3-carboxylic Acid
Molecular Formula
C14H15NO4
Formula Weight
261.3
Purity
≥95%
Emission
472 nm
Excitation
407 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 2 mg/mlDMSO: 2 mg/mlEthanol: insolPBS (pH 7.2): insol
λmax
424 nm
SMILES
O=C(C1=CC2=CC=C(N(CC)CC)C=C2OC1=O)O
InChi Code
InChI=1S/C14H15NO4/c1-3-15(4-2)10-6-5-9-7-11(13(16)17)14(18)19-12(9)8-10/h5-8H,3-4H2,1-2H3,(H,16,17)
InChi Key
WHCPTFFIERCDSB-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    50 mg

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    Product Description

    7-(Diethylamino)coumarin-3-carboxylic acid is a fluorescent probe.1,2 It has been used as a FRET acceptor conjugated to neomycin B or tobramycin (Item No. 14596) for use in the analysis of bacterial RNA binding.1 It has also been used as a reactive probe for the derivatization of sulfonamides for laser-induced fluorescent (LIF) detection.2 7-(Diethylamino)coumarin-3-carboxylic acid displays excitation/emission maxima of 407/472 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Xie, Y., Dix, A.V., and Tor, Y. FRET enabled real time detection of RNA-small molecule binding. J. Am. Chem. Soc. 131(48), 17605-17614 (2009).

    2. Wu, C., Sun, Y., Wang, Y., et al. 7-(Diethylamino)coumarin-3-carboxylic acid as derivatization reagent for 405 nm laser-induced fluorescence detection: A case study for the analysis of sulfonamides by capillary electrophoresis. Talanta 201, 16-22 (2019).