A fluorogenic substrate for phosphatases
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DiFMUP

Item No. 35500

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
6,8-difluoro-4-methyl-7-(phosphonooxy)-2H-1-benzopyran-2-one
CAS Number
214491-43-7
Synonyms
  • 6,8-difluoro-4-Methylumbelliferyl phosphate
Molecular Formula
C10H7F2O6P
Formula Weight
292.1
Purity
≥95%
Emission
455 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: soluble
SMILES
O=C1OC2=C(C(OP(O)(O)=O)=C(C=C2C(C)=C1)F)F
InChi Code
InChI=1S/C10H7F2O6P/c1-4-2-7(13)17-9-5(4)3-6(11)10(8(9)12)18-19(14,15)16/h2-3H,1H3,(H2,14,15,16)
InChi Key
DZANYXOTJVLAEE-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    1 mg

    $54

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    5 mg

    $149

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    Product Description

    DiFMUP is a fluorogenic substrate for phosphatases.1 Upon enzymatic cleavage by phosphatases, 6,8-difluoro-7-hydroxy-4-methylcoumarin (Item No. 36017) is released, which has an absorption/emission maxima of 358/455 nm, respectively, and its fluorescence can be used to quantify phosphatase activity.2 DiFMUP has been used to detect alkaline phosphatase in isolated rat tibia bone tissue.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Gee, K.R., Sun, W.C., Bhalgat, M.K., et al. Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and β-galactosidases. Anal. Biochem. 273(1), 41-48 (1999).

    2. Gade, T.P., Motley, M.W., Beattie, B.J., et al. Imaging of alkaline phosphatase activity in bone tissue. PLoS One 6(7), e22608 (2011).