P-Selectin/CD62P (human, recombinant)

P-selectin, also known as CD62P, is a glycoprotein and cell adhesion molecule that is encoded by the SELP gene in humans.¹ It is a homodimer and is composed of an N-terminal calcium-dependent lectin domain that recognizes glycoproteins, an EGF-like domain, nine consensus repeats, a transmembrane domain, and an intracellular C-terminal tail. P-selectin is expressed in platelets, endothelial cells, and macrophages, is stored in α-granules of platelets, and localizes to the plasma membrane upon platelet activation.^2,3,1 It also exists as a soluble form that results from alternative splicing of SELP.¹ P-selectin is involved in tethering and rolling of leukocytes during inflammation and tissue healing mediated by binding to P-selectin glycoprotein ligand-1 (PSGL-1), a mucin expressed on the surface of leukocytes.^1,4 In vivo, transgenic mice expressing human SELP exhibit neutrophil recruitment in a model of peritonitis induced by thioglycolate.² Polymorphisms in SELP and serum levels of soluble P-selectin are associated with coronary artery disease.⁵ Cayman’s P-Selectin/CD62P (human, recombinant) protein can be used for cell-based assays. This protein is a disulfide-linked homodimer. The reduced monomer, composed of P-selectin/CD62P (amino acids 1-771) fused to human IgG1 Fc at its C-terminus, consists of 971 amino acids and has a calculated molecular weight of 107 kDa, and a predicted N-terminus of Trp42 after signal peptide cleavage. As a result of glycosylation, the monomer migrates at approximately 130-150 kDa by SDS-PAGE under reducing conditions.

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1. Tvaroška, I., Selvaraj, C., and Koča, J. Selectins-The two Dr. Jekyll and M_r. Hyde faces of adhesion molecules-A review. Molecules 25(12), 2835 (2020).

2. Liu, Z., Miner, J.J., Yago, T., et al. Differential regulation of human and murine P-selectin expression and function in vivo. J. Exp. Med. 207(13), 2975-2987 (2010).

3. Israels, S.J., Gerrard, J.M., Jacques, Y.V., et al. Platelet dense granule membranes contain both granulophysin and P-selectin (GMP-140). Blood 80(1), 143-152 (1992).

4. McEver, R.P. Selectins: Initiators of leucocyte adhesion and signalling at the vascular wall. Cardiovasc. Res. 107(3), 331-339 (2015).

5. Barbaux, S.C., Blankenberg, S., Rupprecht, H.J., et al. Association between P-selectin gene polymorphisms and soluble P-selectin levels and their relation to coronary artery disease. Arterioscler. Thromb. Vasc. Biol. 21(10), 1668-1673 (2001).