Host: E. coli • AA: 1-147 (full length) • Tag: C-terminal His • MW: 17.7 kDa
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FHIT (human, recombinant)

Item No. 35510

Product Insert (PDF)
Technical Information
Synonyms
  • Ap3Aase
  • Ap3A Hydrolase
  • Bis(5'-adenosyl)-Triphosphatase
  • Diadenosine 5',5"-P1,P3-Triphosphate Hydrolase
  • Dinucleosidetriphosphatase
  • FRA3B
  • Fragile Histidine Triad Protein
Purity
≥85% estimated by SDS-PAGE
Source
Recombinant human C-terminal His-tagged FHIT expressed in E. coli
Amino Acids
1-147 (full length)
MW
17.7 kDa
Formulation (Request formulation change)
Lyophilized from sterile 50 mM Tris, pH 8.0, with 10% glycerol
UniProt Accession №
P49789
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $240

    Add
    Cayman Chemical
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    Product Description

    Fragile histidine triad diadenosine triphosphatase (FHIT) is a hydrolase and tumor suppressor.1,2,3 It contains a His-x-His-x-His motif that hydrolyzes diadenosine triphosphate (Ap3A), as well as Ap4A and ATP.1 FHIT is expressed in spleen, brain, kidney, lung, and liver and localizes to the plasma membrane, nucleus, mitochondria, and cytoplasm.4,5,6 It is involved in tumor suppression and genome stability.2 Overexpression of FHIT induces apoptosis in lung cancer cells and reduces tumor growth in lung cancer mouse xenograft models.7 Homozygous or heterozygous loss of FHIT expression is prevalent in several cancers, and the FHIT gene contains a common fragile site, FRA3B, that is a translocation breakpoint associated with hereditary kidney cancer.3 Cayman’s FHIT (human, recombinant) protein consists of 153 amino acids and has a calculated molecular weight of 17.7 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 18 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lima, C.D., Klein, M.G., and Hendrickson, W.A. Structure-based analysis of catalysis and substrate definition in the HIT protein family. Science 278(5336), 286-290 (1997).

    2. Waters, C.E., Saldivar, J.C., Hosseini, S.A., et al. The FHIT gene product: Tumor suppressor and genome "caretaker". Cell. Mol. Life Sci. 71(23), 4577-4587 (2014).

    3. Pekarsky, Y., Palamarchuk, A., Huebner, K., et al. FHIT as tumor suppressor: Mechanisms and therapeutic opportunities. Cancer Biol. Ther. 1(3), 232-236 (2002).

    4. Golebiowski, F., Kowara, R., and Pawelczyk, T. Distribution of Fhit protein in rat tissues and its intracellular localization. Mol. Cell. Biochem. 266(1-2), 49-55 (2001).

    5. Costas, M.J., Cameselle, J.C., and Sillero, A. Mitochondrial location of rat liver dinucleoside triphosphatase. The Journal of Biological Chemisty 261(5), 2064-2067 (1986).

    6. Sillero, M.A., Villalba, R., Moreno, A., et al. Dinucleosidetriphosphatase from rat liver. Purification and properties. Eur. J. Biochem. 76(2), 331-337 (1977).

    7. Ji, L., Fang, B., Yen, N., et al. Induction of apoptosis and inhibition of tumorigenicity and tumor growth by adenovirus vector-mediated fragile histidine triad (FHIT) gene overexpression. Cancer Res. 59(14), 3333-3339 (1999).