RNase H2B (C-Term) Rabbit Monoclonal Antibody (Clone RM433)

RNase H2B is a non-catalytic accessory subunit of RNase H2, the major nuclear enzyme responsible for degrading RNA/DNA hybrids and hydrolyzing ribonucleotides misincorporated during genomic replication.^1,2,3 It is localized to nuclear replication foci, where it forms a complex with RNase H2A and RNase H2C, and contains a C-terminal PIP-box motif that meditates its interaction with proliferating cell nuclear antigen (PCNA), which confirms strand specificity to RNase H2.³ Biallelic mutations in RNASEH2B induce Aicardi-Goutières syndrome (AGS), an autoimmune disorder characterized by constitutive activation of cGAS and IFN overproduction.⁴ Mutations in RNASEH2B are also associated with uncomplicated hereditary spastic paraplegia.⁵ Cayman’s RNase H2B (C-Term) Rabbit Monoclonal Antibody (Clone RM433) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Reijns, M.A.M., Bubeck, D., Gibson, L.C.D., et al. The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease. The Journal of Biological Chemisty 286(12), 10530-10539 (2011).

2. Chon, H., Vassilev, A., DePamphilis, M.L., et al. Contributions of the two accessory subunits, RNASEH2B and RNASEH2C, to the activity and properties of the human RNase H2 complex. Nucleic Acids Res. 37(1), 96-110 (2009).

3. Bubeck, D., Reijns, M.A.M., Graham, S.C., et al. PCNA directs type 2 RNase H activity on DNA replication and repair substrates. Nucleic Acids Res. 39(9), 3652-3666 (2011).

4. Feng, S., and Cao, Z. Is the role of human RNase H2 restricted to its enzyme activity? Prog. Biophys. Mol. Biol. 121(1), 66-73 (2016).

5. Spagnoli, C., Frattini, D., Salerno, G.G., et al. RNASEH2B pathogenic gene variant in uncomplicated hereditary spastic paraplegia: Report of a new patient. Neuropediatrics 49(6), 419 (2018).