For immunochemical detection of Pax8
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Pax8 (C-Term) Rabbit Monoclonal Antibody (Clone RM436)

Item No. 35615

Product Insert (PDF)
Technical Information
Synonyms
  • Paired Box Protein 8
Immunogen
A peptide from the C-terminal region of human Pax8
Clone Designation
RM436
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC
Cross Reactivity
(+) Pax8
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    Paired box (Pax) protein 8 (Pax8) is a transcription factor and member of the Pax family that functions as both a transcriptional activator and repressor and has an essential role in thyroid development.1,2 It is composed of a DNA-binding paired domain, conserved octapeptide, partial homeodomain, transactivation domain, and an inhibitory domain.1,3 Alternative splicing of PAX8 pre-mRNA encoding the C-terminus results in five isoforms with varying DNA-binding capabilities and transcriptional activities.4 Pax8 is expressed during the mesenchymal-to-epithelial transition in the development of various organs and localizes to the nucleus. It is involved in cell survival, differentiation, and stem cell lineage commitment.1 Knockout of Pax8 produces smaller thyroids without follicles in mice.5 PAX8 mutations have been found in patients with thyroid dysgenesis, and chromosomal translocations leading to the fusion of PAX8 with PPARG, resulting in expression of Pax8-PPARγ fusion proteins, are associated with follicular thyroid carcinomas.2,6 Cayman's Pax8 (C-Term) Rabbit Monoclonal Antibody (Clone RM436) can be used for immunohistochemistry (IHC).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Robson, E.J.D., He, S.-J., and Eccles, M.R. A PANorama of PAX genes in cancer and developement. Nat. Rev. Cancer 6(1), 52-62 (2006).

    2. Macchia, P.E., Lapi, P., Krude, H., et al. PAX8 mutations associated with congenital hypothyroidism caused by thyroid dysgenesis. Nat. Genet. 19(1), 83-86 (1998).

    3. Poleev, A., Okladnova, O., Musti, A.M., et al. Determination of functional domains of the human transcription factor PAX8 responsible for its nuclear localization and transactivating potential. Eur. J. Biochem. 247(3), 860-869 (1997).

    4. Poleev, A., Wendler, F., Fickenscher, H., et al. Distinct functional properties of three human paired-box-protein, PAX8, isoforms generated by alternative splicing in thyroid, kidney and Wilms' tumors. Eur. J. Biochem. 228(3), 899-911 (1995).

    5. Mansouri, A., Chowdhury, K., and Gruss, P. Follicular cells of the thyroid gland require Pax8 gene function. Nat. Genet. 19(1), 87-90 (1998).

    6. Kroll, T.G., Sarraf, P., Pecciarini, L., et al. PAX8-PPARγ1 fusion in oncogene human thyroid carcinoma. Science 289(5483), 1357-1360 (2000).