A fluorescent dye
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Nucleic Acid Dye Green I

Item No. 35669

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-[[3-(dimethylamino)propyl]propylamino]-4-[(3-methyl-2(3H)-benzothiazolylidene)methyl]-1-phenyl-quinolinium, monochloride
CAS Number
2225748-05-8
Synonyms
  • Green I Nucleic Acid Gel Stain
  • NucGel Green I Nucleic Acid Gel Stain
Molecular Formula
C32H37N4S • Cl
Formula Weight
545.2
Purity
≥95%
Emission
520 nm
Excitation
497 nm
Formulation (Request formulation change)
A solution in DMSO (10,000X)
Solubility(Learn about Variance in Solubility)
DMSO: Soluble
SMILES
CCCN(C1=[N+](C2=CC=CC=C2)C(C=CC=C3)=C3C(C=C(SC4=CC=CC=C54)N5C)=C1)CCCN(C)C.[Cl-]
InChi Code
InChI=1S/C32H37N4S.ClH/c1-5-20-35(22-13-21-33(2)3)31-23-25(24-32-34(4)29-18-11-12-19-30(29)37-32)27-16-9-10-17-28(27)36(31)26-14-7-6-8-15-26;/h6-12,14-19,23-24H,5,13,20-22H2,1-4H3;1H/q+1;/p-1
InChi Key
IHHRNZWXTFDDLM-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    500 µl

    $193

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    Product Description

    Nucleic Acid Dye Green I is a cell-impermeable fluorescent dye that has commonly been used for the quantification of dsDNA and ssDNA.1 It displays excitation and emission maxima at 497 and 520 nm, respectively. Nucleic Acid Dye Green I has also been used in gene expression and RT-PCR assays.2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Suzuki, T., Matsuzaki, T., Hagiwara, H., et al. Recent advances in fluorescent labeling techniques for fluorescence microscopy. Acta Histochem. Cytochem. 40(5), 131-137 (2007).

    2. Ramos-Payán, R., Aguilar-Medina, M., Estrada-Parra, S., et al. Quantification of cytokine gene expression using an economical real-time polymerase chain reaction method based on SYBR® Green I. Scand. J. Immunol. 57(5), 439-445 (2003).

    3. Ponchel, F., Toomes, C., Bransfield, K., et al. Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions. BMC Biotechnol. 3, 18 (2003).