A cell-permeable fluorogenic cell viability probe
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Fluorescein diacetate

Item No. 35701

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3′,6′-bis(acetyloxy)-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
CAS Number
596-09-8
Synonyms
  • 3′,6′-Diacetylfluorescein
  • FDA
  • MFCD 5062
  • NSC 4726
  • NSC 667259
Molecular Formula
C24H16O7
Formula Weight
416.4
Purity
≥98%
Emission
520 nm
Excitation
490 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 5 mg/mlDMSO: 1 mg/ml
λmax
220 nm
SMILES
O=C1OC2(C3=C1C=CC=C3)C4=CC=C(C=C4OC5=CC(OC(C)=O)=CC=C52)OC(C)=O
InChi Code
InChI=1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
InChi Key
CHADEQDQBURGHL-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    5 g

    $70

    Add

    10 g

    $118

    Add

    25 g

    $222

    Add
    Product Description

    Fluorescein diacetate is a cell-permeable fluorogenic probe to quantify cell viability.1 It is cleaved by intracellular esterases to produce fluorescein which displays excitation/emission maxima of 490/520 nm, respectively.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Brunius, G. Technical aspects of the use of 3',6'-diacetyl fluorescein for vital fluorescent staining of bacteria. Curr. Microbiol. 4, 321-323 (1980).

    2. Dive, C., Cox, H., Watson, J.V., et al. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases. Mol.Cell Probe 2(2), 131-145 (1988).