L-Selectin/CD62L Extracellular Domain (mouse, recombinant)

L-Selectin, also known as CD62L, is a glycoprotein and cell adhesion molecule that is encoded by the SELL gene in humans.^1,2 It is composed of an N-terminal calcium-dependent lectin domain that recognizes glycoproteins, an EGF-like domain, two consensus repeats, a transmembrane domain, and an intracellular C-terminal tail. L-selectin is expressed in leukocytes and localizes to the plasma membrane.³ It also exists as a soluble form that results from alternative splicing of SELL pre-mRNA or by ectodomain shedding via proteolytic cleavage.² L-selectin facilitates leukocyte rolling on activated vascular endothelium and lymphocyte homing to high endothelial venules of lymph nodes.⁴ It binds to several glycoproteins containing the sulfated sialyl Lewis X (6-sulfo-sLe^X) epitope, including P-selectin glycoprotein ligand-1 (PSGL-1) and glycosylation-dependent cell adhesion molecule-1 (GlyCAM-1).¹ Sell^-/- mice exhibit a reduction in the lymphocyte count in peripheral lymph nodes, as well as a decrease in neutrophil recruitment in a model of peritonitis induced by thioglycolate.⁴ Serum levels of soluble L-selectin are lower in patients with sepsis and associated with higher mortality.⁵ Cayman’s L-Selectin/CD62L Extracellular Domain (mouse, recombinant) protein can be used for cell-based assays. This protein is a disulfide-linked homodimer. The reduced monomer, composed of L-selectin (amino acids 1-332) fused to His-tagged human IgG1 at its C-terminus, consists of 542 amino acids, has a calculated molecular weight of 61 kDa, and a predicted N-terminus of Trp39 after signal peptide cleavage. As a result of glycosylation, the monomer migrates at approximately 100-110 kDa by SDS-PAGE under reducing conditions.

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1. Tvaroška, I., Selvaraj, C., and Koča, J. Selectins-The two Dr. Jekyll and M_r. Hyde faces of adhesion molecules-A review. Molecules 25(12), 2835 (2020).

2. Ivetic, A., Green, H.L.H., and Hart, S.J. L-selectin: A major regulator of leukocyte adhesion, migration and signaling. Front. Immunol. 10, 1068 (2019).

3. Stein, J.V., Cheng, G., Stockton, B.M., et al. L-selectin-mediated leukocyte adhesion in vivo: Microvillous distribution determines tethering efficiency, but not rolling velocity. J. Exp. Med. 189(1), 37-50 (1999).

4. Arbonés, M.L., Ord, D.C., Ley, K., et al. Lymphocyte homing and leukocyte rolling and migration are impaired in L-selectin-deficient mice. Immunity 1(4), 247-260 (1994).

5. Seidelin, J.B., Nielsen, O.H., and Strøm, J. Soluble L-selectin levels predict survival in sepsis. Intensive Care Med. 28(11), 1613-1618 (2002).