A cell-permeable fluorescent calcium indicator
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Fluo-2 AM

Item No. 35758

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[4-[6-[(acetyloxy)methoxy]-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-glycine (acetyloxy)methyl ester
CAS Number
1070771-36-6
Synonyms
  • Fluo-2 Acetoxymethyl ester
  • Fluo-2 HA
  • Fluo-2 High Affinity
Molecular Formula
C51H52N2O23
Formula Weight
1061.0
Purity
≥95%
Emission
515 nm
Excitation
490 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Soluble
λmax
254 nm
SMILES
O=C1C=CC2=C(C3=CC(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C3)C5=C(C=C(C=C5)OCOC(C)=O)OC2=C1
InChi Code
InChI=1S/C51H52N2O23/c1-30-7-13-41(52(21-47(60)72-26-67-32(3)55)22-48(61)73-27-68-33(4)56)45(17-30)64-15-16-65-46-18-36(8-14-42(46)53(23-49(62)74-28-69-34(5)57)24-50(63)75-29-70-35(6)58)51-39-11-9-37(59)19-43(39)76-44-20-38(10-12-40(44)51)71-25-66-31(2)54/h7-14,17-20H,15-16,21-29H2,1-6H3
InChi Key
ZGEIIQJBYATNMH-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    20 x 50 µg

    $183

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    1 mg

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    Product Description

    Fluo-2 AM is a cell-permeable fluorescent calcium indicator.1,2 It has been used to measure the activity of the neuropeptide Y4 receptor, as well as to detect intracellular calcium in tetrandine-stimulated primary rabbit corpus cavernosum smooth muscle cells. It binds to calcium (Kd = 290 nM) and displays excitation/emission maxima of 490/515 nm, respectively. Fluo-2 AM is also available in a cell-impermeable form (Item No. 35764).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sliwoski, G., Schubert, M., Stichel, J., et al. Discovery of small-molecule modulators of the human Y4 receptor. PLoS One 11(6), e0157146 (2016).

    2. Liu, J.-H., Chen, J., Wang, T., et al. Effects of tetrandrine on cytosolic free calcium concentration in corpus cavernosum smooth muscle cells of rabbits. Asian J. Androl. 8(4), 405-409 (2006).