A fluorometric assay for the detection of intracellular calcium flux
Features
  • No wash ratiometric calcium (Ca2+) assay with adherent or non-adherent cells
  • Compatible Instruments: Fluorescence microscope, plate reader
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Ratiometric Calcium Assay Kit

Item No. 35761

Kit Booklet (PDF)
Technical Information
Emission
505 nm (Ca2+-bound Fura-2); 505 nm (Ca2+-free Fura-2)
Excitation
340 nm (Ca2+-bound Fura-2); 380 nm (Ca2+-free Fura-2)
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    This Ratiometric Calcium Assay Kit provides the reagents necessary to perform no-wash, ratiometric Ca2+ flux assays compatible with fluorescence plate readers. Individual components are provided to allow flexibility in optimizing assay conditions for different cell types and experimental systems.

    When prepared according to the recommended protocol, this kit provides sufficient reagents to generate approximately 100 ml of dye loading solution, which is enough to process ten 96-well plates when using 100 μl per well. The total number of assays may vary depending on the concentration and experimental conditions required for a given application.

    WARNING This product is not for human or veterinary use.