For immunochemical detection of granzyme B
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Granzyme B (C-Term) Rabbit Monoclonal Antibody (Clone RM441)

Item No. 35806

Product Insert (PDF)
Technical Information
Synonyms
  • CTLA-1
  • Cytotoxic Serine Protease B
  • Cytotoxic T-lymphocyte Proteinase 2
  • Fragmentin-2
  • Granzyme-2
  • GrB
  • GZMB
Immunogen
Peptide from the C-terminal region of granzyme B
Clone Designation
RM441
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC
Cross Reactivity
(+) Granzyme B
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    Granzyme B is a serine protease that is involved in cytotoxic T lymphocyte- and natural killer cell-induced cell death.1,2 It localizes to cytoplasmic granules prior to release into the immunological synapse formed with a target cell.3,1 Granzyme B cleaves its substrates after an aspartate residue and targets caspase-3, caspase-8, and gasdermin E (GSDME), among others.1,2 It is involved in caspase-dependent and -independent apoptosis, necrosis, DNA fragmentation, pyroptosis, viral infection, and antitumor immunity.1,2,4 Knockout of the gene encoding granzyme B, GZMB, reduces disease severity in a mouse model of epidermolysis bullosa acquisita, an autoimmune disease characterized by blistering of the skin.5 Serum and synovial levels of granzyme B are elevated in patients with rheumatoid arthritis.6 Cayman’s Granzyme B (C-Term) Rabbit Monoclonal Antibody (Clone RM441) can be used for immunohistochemistry (IHC).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Barry, M., and Bleackley, R.C. Cytotoxic T lymphocytes: All roads lead to death. Nat. Rev. Immunol. 2(6), 401-409 (2002).

    2. Tsuchiya, K. Switching from apoptosis to pyroptosis: Gasdermin-elicited inflammation and antitumor immunity. Int. J. Mol. Sci. 22(1), 426 (2021).

    3. Pinkoski, M.J., Hobman, M., Heibein, J.A., et al. Entry and trafficking of granzyme B in target cells during granzyme B-perforin-mediated apoptosis. Blood 92(3), 1044-1054 (1998).

    4. Trapani, J.A., and Sutton, V.R. Granzyme B: Pro-apoptotic, antiviral and antitumor functions. Curr. Opin. Immunol. 15(5), 533-543 (2003).

    5. Hiroyasu, S., Zeglinski, M.R., Zhao, H., et al. Granzyme B inhibition reduces disease severity in autoimmune blistering diseases. Nat. Commun. 12(1), 302 (2021).

    6. Goldbach-Mansky, R., Suson, S., Wesley, R.A., et al. Raised granzyme B levels are associated with erosions in patients with early rheumatoid factor positive rheumatoid arthritis. Ann. Rheum. Dis. 64(5), 715-721 (2005).