Δ² α-Tubulin Rabbit Monoclonal Antibody (Clone RM447)

Δ² α-Tubulin is a non-tyrosinatable variant of tubulin that lacks a carboxy-terminal glutamyl-tyrosine group on its α-subunit.^1,2 It is expressed in a wide variety of cell types, including fibroblasts and adrenal cortical cells, and accounts for approximately 35% of brain tubulin. Δ² α-Tubulin is primarily localized to stable cellular structures such as centrosomes and primary cilia, however, it localizes to microtubule bundles and paracrystalline bundles following cellular exposure to paclitaxel or vinblastine, respectively.¹ It is also subject to post-translational modifications, including glutamylation, and levels of glutamylated Δ² α-tubulin are increased in mouse models of acute epileptic seizure and slow-developing Alzheimer’s disease.³ Cayman’s Δ² α-Tubulin Rabbit Monoclonal Antibody (Clone RM447) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Paturle-Lafanechère, L., Manier, M.L., Trigault, N., et al. Accumulation of delta 2-tubulin, a major tubulin variant that cannot be tyrosinated, in neuronal tissues and in stable microtubule assemblies. J. Cell Sci. 107(Pt 6), 1529-1543 (1994).

2. Paturle, L., Wehland, J., Margolis, R.L., et al. Complete separation of tyrosinated, detyrosinated, and nontyrosinatable brain tubulin subpopulations using affinity chromatography. Biochemistry 28(6), 2698-2704 (1989).

3. Vi, H.T., Akatsu, H., Hashizume, Y., et al. Increase in α-tubulin modifications in the neuronal processes of hippocampal neurons in both kainic acid-induced epileptic seizure and Alzheimer’s disease. Sci. Rep. 7, 40205 (2017).