A reducing agent
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

DL-Dithiothreitol

Item No. 35885

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
rel-1,4-dimercapto-2R,3R-butanediol
CAS Number
3483-12-3
Synonyms
  • DTT
  • Cleland’s Reagent
  • WR 34678
Molecular Formula
C4H10O2S2
Formula Weight
154.3
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Acetonitrile: SolubleDMSO: SolubleWater: Soluble
SMILES
SC[C@@H](O)[C@H](O)CS
InChi Code
InChI=1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChi Key
VHJLVAABSRFDPM-QWWZWVQMSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 g

    $28

    Add

    5 g

    $104

    Add

    10 g

    $193

    Add

    25 g

    $310

    Add
    Product Description

    DL-Dithiothreitol is a reducing agent.1,2 It has been used to stabilize thiols in a reduced state in various applications, including protein purification and enzymatic assays.3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cleland, W.W. Dithiothreitol, a new protective reagent for SH groups. Biochemistry 3, 480-482 (1964).

    2. Muller, T., and Winter, D. Systematic evaluation of protein reduction and alkylation reveals massive unspecific side effects by iodine-containing reagents. Mol. Cell Proteomics 16(7), 1173-1187 (2017).

    3. Getz, E.B., Xiao, M., Chakrabarty, T., et al. A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry. Anal. Biochem. 273(1), 73-80 (1999).

    4. Avdic, V., Zhang, P., Lanouette, S., et al. Structural and biochemical insights into MLL1 core complex assembly. Structure 19(1), 101-108 (2011).