For immunochemical detection of JNK1/2/3 (phospho-Thr183/Tyr185)
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JNK1/2/3 (Phospho-Thr183/Tyr185) Rabbit Monoclonal Antibody (Clone RM464)

Item No. 35906

Product Insert (PDF)
Technical Information
Synonyms
  • c-Jun N-terminal Kinase
Immunogen
Peptide corresponding to human JNK1/2/3 (phospho-Thr183/Tyr185)
Clone Designation
RM464
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) JNK1/2/3 (phospho-Thr183/Tyr185(-) JNK1/2/3 without phosphorylation at Thr183/Tyr185
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    Cayman Chemical
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    Product Description

    c-Jun N-terminal kinases (JNKs) are stress-activated serine/threonine protein kinases and members of the MAPK family with roles in cell proliferation, differentiation, apoptosis, and migration.1,2 JNKs are encoded by three distinct genes, JNK1, JNK2, and JNK3, which produce 10 variants via alternative splicing. JNK1 and JNK2 are ubiquitously expressed while JNK3 is expressed at high levels in the CNS and low levels in cardiac smooth muscle and the testis.1 In response to stress stimuli, such as osmotic or UV shock, cytokines, or growth factor signaling, JNKs are activated by phosphorylation at threonine 183 (Thr183) and tyrosine 185 (Tyr185) by MKK7 and MKK4, respectively, in a synergistic manner, and induce various transcription factor-mediated downstream signaling events.3,1 Neuronal levels of JNK (phospho- Thr183/Tyr185) are increased in mouse models of sporadic and prion-induced Alzheimer's disease.4 Renal JNK (phospho- Thr183/Tyr185) levels are increased in patients with glomerulonephritis and diabetic neuropathy and are associated with the degree of glomerulosclerosis and macrophage infiltration.2 Cayman's JNK1/2/3 (Phospho-Thr183/Tyr185) Rabbit Monoclonal Antibody (Clone RM464) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kumar, A., Singh, U.K., Kini, S.G., et al. JNK pathway signaling: A novel and smarter therapeutic target for various biological diseases. Future Med. Chem. 7(15), 2065-2086 (2015).

    2. Grynberg, K., Ma, F.Y., and Nikolic-Paterson, D.J. The JNK signaling pathway in renal fibrosis. Front. Physiol. 8(829), (2017).

    3. Thévenin, A.F., Zony, C.L., Bahnson, B.J., et al. Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts. Protein Expr. Purif. 75(2), 138-146 (2011).

    4. Okazawa, H., and Estus, S. The JNK/c-Jun cascade and Alzheimer’s disease. Am. J. Alzheimers Dis. Other Demen. 17(2), 79-88 (2002).