A fluorophore
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6,8-Difluoro-7-hydroxy-4-methylcoumarin

Item No. 36017

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
6,8-difluoro-7-hydroxy-4-methyl-2H-1-benzopyran-2-one
CAS Number
215868-23-8
Molecular Formula
C10H6F2O3
Formula Weight
212.2
Purity
≥98%
Emission
455 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: solubleDMSO: solubleMethanol: soluble
λmax
320 nm
SMILES
O=C1OC2=C(C(O)=C(C=C2C(C)=C1)F)F
InChi Code
InChI=1S/C10H6F2O3/c1-4-2-7(13)15-10-5(4)3-6(11)9(14)8(10)12/h2-3,14H,1H3
InChi Key
LLENVBUPWUQAGL-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $32

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    5 mg

    $102

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    10 mg

    $172

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    25 mg

    $349

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    Product Description

    6,8-Difluoro-7-hydroxy-4-methylcoumarin is a fluorophore.1 It has been conjugated to streptavidin or secondary antibodies for flow cytometry and used to label bacteria for multi-color fluorescence in situ hybridization (FISH).2,1 6,8-Difluoro-7-hydroxy-4-methylcoumarin has also been coupled to peptides to improve electrospray ionization and MS sensitivity in MALDI-TOF MS.3 It displays absorption/emission maxima of 358/455 nm, respectively.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Takada, T., Matsumoto, K., and Nomoto, K. Development of multi-color FISH method for analysis of seven Bifidobacterium species in human feces. J. Microbiol. Methods 58(3), 413-421 (2004).

    2. Telford, W.G. Analysis of UV-excited fluorochromes by flow cytometry using near-ultraviolet laser diodes. Cytometry A. 61(1), 9-17 (2004).

    3. Pashkova, A., Moskovets, E., and Karger, B.L. Coumarin tags for improved analysis of peptides by MALDI-TOF MS and MS/MS. 1. Enhancement in MALDI MS signal intensities. Anal. Chem. 76(15), 4550-4557 (2004).

    4. Sun, W.C., Gee, K.R., and Haugland, R.P. Synthesis of novel fluorinated coumarins: Excellent UV-light excitable fluorescent dyes. Bioorg. Med. Chem. Lett. 8(22), 3107-3110 (1998).