A fluorescent probe for albumin and CAII
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Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes

Research Area

Application

Fluorescence

Dansyl Amide

Item No. 36149

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-(dimethylamino)-1-naphthalenesulfonamide
CAS Number
1431-39-6
Synonyms
  • DNSA
Molecular Formula
C12H14N2O2S
Formula Weight
250.3
Purity
≥98%
Emission
470 nm
Excitation
280 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 2 mg/mlDMSO: 1 mg/ml
λmax
216, 249 nm
SMILES
O=S(N)(C1=CC=CC2=C1C=CC=C2N(C)C)=O
InChi Code
InChI=1S/C12H14N2O2S/c1-14(2)11-7-3-6-10-9(11)5-4-8-12(10)17(13,15)16/h3-8H,1-2H3,(H2,13,15,16)
InChi Key
TYNBFJJKZPTRKS-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    500 mg

    $40

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    1 g

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    5 g

    $231

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    Product Description

    Dansyl amide is a fluorescent probe for albumin and CAII.1,2 It binds to serum albumin and carbonic anhydrase II (CAII; Kds = 7.57 and 0.2 µM, respectively) and has been used to determine ligand affinities for these proteins. Dansyl amide binding to CAII results in an increase in fluorescence due to FRET from nearby active site tryptophan residues and displays excitation/emission maxima of 280/470 nm, respectively, when bound to CAII and a decrease in fluorescence can be used to quantify CAII ligand binding.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Epps, D.E., Raub, T.J., and Kézdy, F.J. A general, wide-rage spectrofluorometric method for measuring the site-specific affinities of drugs toward human serum albumin. Anal. Biochem. 227(2), 342-350 (1995).

    2. Wang, S.C., and Zamble, D.B. Fluorescence analysis of sulfonamide binding to carbonic anhydrase. Biochem. Mol. Biol. Educ. 34(5), 364-368 (2006).