A colorimetric substrate for β-D-galactosidase
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p-Nitrophenyl β-D-Galactopyranoside

Item No. 36160

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-nitrophenyl β-D-galactopyranoside
CAS Number
3150-24-1
Synonyms
  • p-Nitrophenyl β-D-galactoside
  • pNPGal
  • NSC 89287
  • PNPG
Molecular Formula
C12H15NO8
Formula Weight
301.3
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 11 mg/mlDMSO: 10 mg/mlEthanol: Slightly solublePBS (pH 7.2): 0.09 mg/ml
λmax
220, 303 nm
SMILES
O[C@H]([C@H]([C@H]([C@H](O1)CO)O)O)[C@@H]1OC2=CC=C(C=C2)[N+]([O-])=O
InChi Code
InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1
InChi Key
IFBHRQDFSNCLOZ-YBXAARCKSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    500 mg

    $44

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    1 g

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    5 g

    $260

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    10 g

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    Product Description

    p-Nitrophenyl β-D-galactopyranoside is a chromogenic substrate for β-D-galactosidase.1 Upon enzymatic cleavage by β-D-galactosidase, p-nitrophenol is released, which can be quantified by colorimetric detection at 420 nm as a measure of β-D-galactosidase activity. p-Nitrophenyl β-D-galactopyranoside has been used to determine the substrate specificity of T. maritima MSB8 β-galactosidase.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Darwish, I.A., Mahmoud, A.M., and Al-Majed, A.-R.A. Generic simple enzyme immunoassay approach to avert small molecule immobilization problems on solid phases: Application to the determination of tobramycin in serum. Talanta 72(4), 1322-1328 (2007).

    2. Li, L., Zhang, M., Jiang, Z., et al. Characterisation of a thermostable family 42 β-galactosidase from Thermotoga maritima. Food Chem. 112, 844-850 (2009).