A colorimetric chymotrypsin substrate
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Chymotrypsin Substrate I, Colorimetric (trifluoroacetate salt)

Item No. 36194

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-(3-carboxy-1-oxopropyl)glycylglycyl-N-(4-nitrophenyl)-L-phenylalaninamide, trifluoroacetate salt
Synonyms
  • Suc-GGF-pNA
  • Suc-Gly-Gly-Phe-pNA
  • Suc-Gly-Gly-Phe-p-Nitroanilide
Molecular Formula
C23H25N5O8 • XCF3COOH
Formula Weight
499.5
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 5 mg/mlDMSO: 10 mg/mlEthanol: insolPBS (pH 7.2): insol
SMILES
O=C(NC(C=C1)=CC=C1[N+]([O-])=O)[C@@H](NC(CNC(CNC(CCC(O)=O)=O)=O)=O)CC2=CC=CC=C2.OC(C(F)(F)F)=O
InChi Code
InChI=1S/C23H25N5O8.C2HF3O2/c29-19(10-11-22(32)33)24-13-20(30)25-14-21(31)27-18(12-15-4-2-1-3-5-15)23(34)26-16-6-8-17(9-7-16)28(35)36;3-2(4,5)1(6)7/h1-9,18H,10-14H2,(H,24,29)(H,25,30)(H,26,34)(H,27,31)(H,32,33);(H,6,7)/t18-;/m0./s1
InChi Key
NOLWOQGDNMFZTI-FERBBOLQSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $37

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    5 mg

    $108

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    10 mg

    $198

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    25 mg

    $404

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    Product Description

    Chymotrypsin substrate I, colorimetric is a colorimetric substrate for chymotrypsin.1 Chymotrypsin binds and hydrolyzes chymotrypsin substrate I, colorimetric to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of chymotrypsin activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kasafírek, E., and Bartík, M. N-Acylated phenylalanine p-nitroanilides: New substrates for chymotrypsin. Collect. Czech. Chem. Commun. 45, 442-451 (1980).