A colorimetric substrate for γ-glutamyl transpeptidase
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L-Glutamic Acid γ-p-Nitroanilide (hydrate)

Item No. 36209

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-(4-nitrophenyl)-L-glutamine, monohydrate
CAS Number
122864-94-2
Synonyms
  • γ-Glu-pNA
  • Glutamate γ-(4-Nitroanilide)
  • L-GPNA
  • E-pNA
Molecular Formula
C11H13N3O5 • H2O
Formula Weight
285.3
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Chloroform: Slightly solubleDMF: Slightly solubleDMSO: Slightly solubleEthanol: Slightly solubleMethanol: Slightly solublePBS (pH 7.2): Slightly soluble
λmax
223, 315 nm
SMILES
OC([C@@H](N)CCC(NC(C=C1)=CC=C1[N+]([O-])=O)=O)=O.O
InChi Code
InChI=1S/C11H13N3O5.H2O/c12-9(11(16)17)5-6-10(15)13-7-1-3-8(4-2-7)14(18)19;/h1-4,9H,5-6,12H2,(H,13,15)(H,16,17);1H2/t9-;/m0./s1
InChi Key
HAFIAIPYVFZHSY-FVGYRXGTSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

    $90

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    5 g

    $312

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    Product Description

    L-Glutamic acid γ-p-nitroanilide (γ-Glu-pNA) is a colorimetric substrate for γ-glutamyl transpeptidase.1,2 Upon enzymatic cleavage, pNA is released which can be quantified by colorimetric detection at 405 nm as a measure of γ-glutamyl transpeptidase activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Griffith, O.W., and Tate, S.S. The apparent glutathione oxidase activity of γ-glutamyl transpeptidase. Chemical mechanism. The Journal of Biological Chemisty 255(11), 5011-5014 (1980).

    2. King, J.B., West, M.B., Cook, P.F., et al. A novel, species-specific class of uncompetitive inhibitors of γ-glutamyl transpeptidase. The Journal of Biological Chemisty 284(14), 9059-9065 (2009).