A fluorescently tagged form of dUTP
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Fluorescein-12-dUTP

Item No. 36232

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2′-deoxy-5-[3-[[6-[[[3′,6′-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-5(or 6)-yl]carbonyl]amino]-1-oxohexyl]amino]-1-propen-1-yl]-uridine 5′-(tetrahydrogen triphosphate), trisodium salt
Synonyms
  • FITC-12-dUTP
Molecular Formula
C39H38N4O21P3 • 3Na
Formula Weight
1060.6
Purity
≥95%
Emission
517 nm
Excitation
498 nm
Formulation (Request formulation change)
A solution in 1 mM Tris buffer (pH 7.5)
Solubility(Learn about Variance in Solubility)
Water: Soluble
SMILES
O=C(N1)N([C@]2([H])O[C@H](COP(OP(OP(O)([O-])=O)([O-])=O)([O-])=O)[C@@H](O)C2)C=C(C=CCNC(CCCCCNC(C3=CC=C(C(C(O4)=O)=C3)C54C6=CC=C(O)C=C6OC7=CC(O)=CC=C75)=O)=O)C1=O.O=C(C8=CC=C(C(O9)=O)C(C%109C%11=CC=C(O)C=C%11OC%12=CC(O)=CC=C%12%10)=C8)NCCCCCC(NCC=CC%13=CN([C@@]%14([H])C[C@H](O)[C@@H](COP(OP(OP(O)([O-])=O)([O-])=O)([O-])=O)O%14)C(NC%13=O)=O)=O.[Na+].[Na+].[Na+]
InChi Code
InChI=1S/2C39H41N4O21P3.3Na/c44-23-8-11-27-30(16-23)60-31-17-24(45)9-12-28(31)39(27)26-10-7-21(15-25(26)37(50)62-39)35(48)41-13-3-1-2-6-33(47)40-14-4-5-22-19-43(38(51)42-36(22)49)34-18-29(46)32(61-34)20-59-66(55,56)64-67(57,58)63-65(52,53)54;44-23-8-11-26-30(16-23)60-31-17-24(45)9-12-27(31)39(26)28-15-21(7-10-25(28)37(50)62-39)35(48)41-13-3-1-2-6-33(47)40-14-4-5-22-19-43(38(51)42-36(22)49)34-18-29(46)32(61-34)20-59-66(55,56)64-67(57,58)63-65(52,53)54;;;/h2*4-5,7-12,15-17,19,29,32,34,44-46H,1-3,6,13-14,18,20H2,(H,40,47)(H,41,48)(H,55,56)(H,57,58)(H,42,49,51)(H2,52,53,54);;;/q;;3*+1/p-6/t2*29-,32+,34+;;;/m00.../s1
InChi Key
AJPKOGWOVUMCMI-ZSILEFOTSA-H
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    25 nmol

    $217

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    Product Description

    Fluorescein-12-dUTP is a fluorescently tagged form of deoxy-UTP that can be used to generate fluorescently labeled DNA via reverse transcription, nick translation, random primed labeling, or PCR. It displays ex/em maxima of 498/517 nm, respectively. Fluorescein-12-dUTP has been used to detect nucleic acids in situ.1

    As a starting point for labeling, utilize approximately 0.05 µg/µl DNA, 25-50 U/ml DNA polymerase, and 10-100 µM fluorescein-12-dUTP in 10 mM Tris-HCl, pH 7.5, containing 1 mM EDTA, 5 mM NaCl, and 0.1 mM DTT, as well as dATP, dCTP, dGTP, and dTTP at 1 mM each in a total volume of 20 µl.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Koch, J., Mogensen, J., Pedersen, S., et al. Fast one-step procedure for the detection of nucleic acids in situ by primer-induced sequence-specific labeling with fluorescein-12-dUTP. Cytogenet. Cell Genet. 60(1), 1-3 (1992).