A fluorescent compound and an inhibitor of FTO
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5-Aminofluorescein

Item No. 36270

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-amino-3′,6′-dihydroxy-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
CAS Number
3326-34-9
Synonyms
  • 5-AF
  • Fluoresceinamine isomer 1
Molecular Formula
C20H13NO5
Formula Weight
347.3
Purity
≥95%
Emission
515 nm
Excitation
490 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 5 mg/mlDMSO: 5 mg/ml
λmax
226 nm
SMILES
O=C1OC2(C3=CC=C(C=C13)N)C4=CC=C(C=C4OC5=CC(O)=CC=C52)O
InChi Code
InChI=1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2
InChi Key
GZAJOEGTZDUSKS-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    5-Aminofluorescein is a fluorescent compound and an inhibitor of the mRNA N6-methyladenosine (m6A) demethylase fat mass and obesity-associated (FTO) protein (IC50 = 6.55 µM).1,2 It displays excitation/emission maxima of 490 and 515 nm, respectively, and has been conjugated to human serum albumin for fluorescence imaging of glioblastomas.1,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Al-Natour, M.A., Yousif, M.D., Cavanagh, R., et al. Facile dye-initiated polymerization of lactide-glycolide generates highly fluorescent poly(lactic-co-glycolic acid) for enhanced characterization of cellular delivery. ACS Macro Lett. 9(3), 431-437 (2020).

    2. Wang, T., Hong, T., Huang, Y., et al. Fluorescein derivatives as bifunctional molecules for the simultaneous inhibiting and labeling of FTO protein. J. Am. Chem. Soc. 137(43), 13736-13739 (2015).

    3. Ding, R., Frei, E., Fardanesh, M., et al. Pharmacokinetics of 5-aminofluorescein-albumin, a novel fluorescence marker of brain tumors during surgery. J. Clin. Pharmacol. 51(5), 672-678 (2011).