Active • Host: Insect cells • AA: 564-853 (DNMT3B) and 160-387 (DNMT3L) • Tag: N-terminal GST on DNMT3B, N-terminal His on DNMT3L • MW: 59 kDa (DNMT3B) and 27 kDa (DNMT3L)
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DNMT3B/DNMT3L Complex (human, recombinant)

Item No. 36311

Product Insert (PDF)
Technical Information
Synonyms
  • DNA (Cytosine-5)-Methyltransferase 3B
  • DNA (Cytosine-5)-Methyltransferase 3-Like
  • DNA Methyltransferase HsaIIIB
  • DNA MTase HsaIIIB
  • M.HsaIIIB
Purity
≥90% estimated by SDS-PAGE
Source
Active recombinant human N-terminal GST-tagged DNMT3B and N-terminal His-tagged DNMT3L expressed in insect cells
Amino Acids
564-853 (DNMT3B) and 160-387 (DNMT3L)
MW
59 kDa (DNMT3B) and 27 kDa (DNMT3L)
Formulation (Request formulation change)
40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 16 mM glutathione, and 20% glycerol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    10 µg

    $576

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    Product Description

    DNA methyltransferase 3B (DNMT3B) and DNMT3L are members of the DNMT3 family.1,2 DNMT3B is composed of variable N-terminal, proline-tryptophan-tryptophan-proline (PWWP), cysteine-rich, and C-terminal catalytic domains. DNMT3L has a similar sequence to DNMT3B but lacks the PWWP domain and residues in the C-terminal domain that are required for DNA methyltransferase activity.1 DNMT3B and DNMT3A are responsible for de novo methylation of cytosines in cytosine-phosphate-guanine (CpG) sites of DNA in germ cells and embryos and DNMT3L is required for their activity.1,2,3 The methylation patterns established by the DNMT3 family are maintained by DNMT1.2 Mutations in DNMT3B are associated with ICF syndrome, which is characterized by immune defects, centromeric instability, and facial anomalies.1 Cayman’s DNMT3B/DNMT3L Complex (human, recombinant) protein can be used for enzyme assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cheng, X., and Blumenthal, R.M. Mammalian DNA methyltransferases: A structural perspective. Structure 16(3), 341-350 (2008).

    2. Jeltsch, A., and Jurkowska, R.Z. DNA Methyltransferases - Role and Function. Advances in Experimental Medicine and Biology 945, (2016).

    3. Suetake, I., Shinozaki, F., Miyagawa, J., et al. DNMT3L stimulates the DNA methylation activity of Dnmt3a and Dnmt3b through a direct interaction. The Journal of Biological Chemisty 279(26), 27816-27823 (2004).