A dye and brominated fluorescein derivative
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Alternative(s)
40128Eosin Y
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Eosin Y (sodium salt)

Item No. 36335

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
9′-[9H]xanthen]-3-one, 2′,4′,5′,7′-tetrabromo-3′,6′-dihydroxy-spiro[isobenzofuran-1(3H), disodium salt
CAS Number
17372-87-1
Synonyms
  • C.I. 45380
  • Eosin Yellowish
  • Tetrabromofluorescein
Molecular Formula
C20H6Br4O5 • 2Na
Formula Weight
691.9
Purity
≥95%
Emission
544 nm
Excitation
526 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: 1 mg/ml
λmax
253, 533 nm
SMILES
O=C1OC2(C3=CC(Br)=C(C(Br)=C3OC4=C(C([O-])=C(C=C42)Br)Br)[O-])C5=C1C=CC=C5.[Na+].[Na+]
InChi Code
InChI=1S/C20H8Br4O5.2Na/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20;;/h1-6,25-26H;;/q;2*+1/p-2
InChi Key
LSMXXHPAEZLILP-UHFFFAOYSA-L
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 g

    $43

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    10 g

    $82

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    25 g

    $193

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    50 g

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    Product Description

    Eosin Y is a dye and brominated derivative of the fluorescent compound fluorescein.1 It exists as a tautomer in aqueous solutions.2,1 Eosin Y has minimal pH dependence at physiological pH and has commonly been used in hematoxylin and eosin (H&E) staining to stain the extracellular matrix and cytoplasmic proteins.3,4 It displays absorption/emission maxima of 526/544 nm, respectively.5 It has also been used as a photoredox catalyst in organic synthesis reactions, including for cyclization and the formation of carbon-carbon or carbon-non-carbon bonds, and as a cooperative catalyst with transition metals.1 Eosin Y (100 µM) non-selectively inhibits histone methyltransferases.6 Formulations containing eosin Y have been used in the screening of cervical cancer as part of the Papanicolaou (Pap) staining process.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bosveli, A., Montagnon, T., Kalaitzakis, D., et al. Eosin: A versatile organic dye whose synthetic uses keep expanding. Org. Biomol. Chem. 19(15), 3303-3317 (2021).

    2. McHedlov-Petrossyan, N.O., Vodolazkaya, N.A., Surov, Y.N., et al. 2,4,5,7-Tetranitrofluorescein in solutions: Novel type of tautomerism in hydroxyxanthene series as detected by various spectral methods. Spectrochim. Acta A Mol. Biomol. Spectrosc. 61(11-12), 2747-2760 (2005).

    3. Slyusareva, E., and Gerasimova, M.A. pH-dependence of the absorption and fluorescent properties of fluorone dyes in aqueous solutions. Russ. Phys. J. 56(12), 1370-1377 (2014).

    4. Fischer, A.H., Jacobson, K.A., Rose, J., et al. Hematoxylin and eosin staining of tissue and cell sections. CSH Protoc. 3(5), prot4986 (2006).

    5. Seybold, P.G., Gouterman, M., and Callis, J. Calorimetric, photometric and lifetime determinations of fluorescence yields of fluorescein dyes. Photochem. Photobiol. 9(3), 229-242 (1969).

    6. Okabe, J., Fernandez, A.Z., Ziemann, M., et al. Endothelial transcriptome in response to pharmacological methyltransferase inhibition. ChemMedChem 9(8), 1755-1762 (2014).