Hinokiflavone

Hinokiflavone is a biflavonoid that has been found in A. dammara and has diverse biological activities.^1,2,3,4 It inhibits the aggregation of amyloid-β (1-40) (Aβ40; Item No. 21617) in vitro (IC₅₀ = 9.5 μM).¹ Hinokiflavone (10 µM) promotes pre-mRNA splicing of the genes encoding myeloid cell leukemia-1 (Mcl-1) and eukaryotic translation initiation factor 4α subunit (eIF4α) and inhibits pre-mRNA splicing of the genes encoding heat shock protein 40 (Hsp40) and RIO kinase 3 (RIOK3) in NB4 cells, as well as increases protein SUMOylation in HEK293 cells.² It has antiviral activity against the influenza A subtypes H1N1 and H1N3, influenza B, parainfluenza type 3, and respiratory syncytial virus (RSV) in cytopathic effect assays (IC₅₀s = 1.4, 1.3, 1.2, 1, and 2.5 µg/ml, respectively).³ Hinokiflavone (10-40 µM) inhibits the migration and invasion of, and induces apoptosis in, KYSE-150 esophageal squamous cell carcinoma (ESCC) cells in vitro and reduces tumor volume in a KYSE-150 mouse xenograft model when administered at doses of 25 or 50 mg/kg.⁴

WARNING This product is not for human or veterinary use.

1. Sirimangkalakitti, N., Juliawaty, L.D., Hakim, E.H., et al. Naturally occurring biflavonoids with amyloid β aggregation inhibitory activity for development of anti-Alzheimer agent. Bioorg. Med. Chem. Lett. 29(15), 1994-1997 (2019).

2. Pawellek, A., Ryder, U., Tammsalu, T., et al. Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP. Elife 6:e27402, (2017).

3. Lin, Y.-M., Flavin, M.T., Schure, R., et al. Antiviral activities of biflavonoids. Planta. Med. 65(2), 120-125 (1999).

4. Gou, J., Zhang, S., Wang, J., et al. Hinokiflavone inhibits growth of esophageal squamous cancer by inducing apoptosis via regulation of the PI3K/AKT/mTOR signaling pathway. Front. Oncol. 12:833719, (2022).