TMPRSS2 Monoclonal Antibody (Clone 3E10)

TMPRSS2 is a type II transmembrane serine protease that has major roles in androgen receptor signaling, viral infection, and carcinogenesis.¹ TMPRSS2 is composed of an N-terminal transmembrane domain and an extracellular stem region that contains an LDL receptor class A (LDLRA) domain that binds calcium, as well as a scavenger receptor cysteine-rich (SRCR) domain that interacts with other extracellular molecules. It is synthesized as a zymogen and the C-terminal serine protease domain undergoes autoproteolytic cleavage, releasing the soluble protease into the extracellular space. TMPRSS2 mRNA is mainly expressed in the prostate gland but is also found in the salivary and mammary glands, colon, stomach, small intestine, kidney, liver, and lung.² Soluble TMPRSS2 cleaves and activates proteinase-activated receptor 2 (PAR2) in prostate cancer cells.³ TMPRSS2 facilitates host cell entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in vitro, and knockout of Tmprss2 in human dipeptidyl peptidase 4-expressing transgenic mice reduces disease severity in a model of Middle East respiratory syndrome coronavirus (MERS-CoV) infection.^4,5 Chromosomal translocations leading to TMPRSS2-ERG fusions are associated with prostate cancer.⁶ Cayman’s TMPRSS2 Monoclonal Antibody (Clone 3E10) can be used for ELISA, immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes TMPRSS2 at approximately 65 kDa from human samples.

WARNING This product is not for human or veterinary use.

1. Thunders, M., and Delahunt, B. Gene of the month: TMPRSS2 (transmembrane serine protease 2). J. Clin. Pathol. 73(12), 773-776 (2020).

2. Vaarala, M.H., Porvari, K.S., Kellokumpu, S., et al. Expression of transmembrane serine protease TMPRSS2 in mouse and human tissues. J. Pathol. 193(1), 134-140 (2001).

3. Wilson, S., Greer, B., Hooper, J., et al. The membrane-anchored serine protease, TMPRSS2, activates PAR-2 in prostate cancer cells. Biochem. J. 388(Pt 3), 967-972 (2005).

4. Hoffmann, M., Kleine-Weber, H., Schroeder, S., et al. SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor. Cell 181(2), 271-280 (2020).

5. Iwata-Yoshikawa, N., Okamura, T., Shimizu, Y., et al. TMPRSS2 contributes to virus spread and immunopathology in the airways of murine models after coronavirus infection. J. Virol. 93(6), e01815-01818 (2019).

6. García-Perdomo, H.A., Chaves, M.J., Osorio, J.C., et al. Association between TMPRSS2:ERG fusion gene and the prostate cancer: Systematic review and meta-analysis. Cent. European J. Urol. 71(4), 410-419 (2018).