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INDIGlo LDR works equally well in quantifying expressed luciferase activity from 96- or 384-well format reporter assays. The resulting light emission is stable over a 2 hr period, thereby making INDIGlo LDR an ideal, economical detection reagent for both low- and high-throughput batch processing of assay plates.
INDIGlo LDR is formulated as a 2x-concentrated reagent, thereby allowing greater versatility in its use. The 2x-concentrated reagent will be used when using 384-well or 1536-well assay plates that are processed using a homogenous assay strategy.
Alternatively, when processing 96-well assay plates either a homogenous or non-homogenous assay strategy may be used. For convenience, this kit provides an optimized Dilution Buffer for use in diluting INDIGlo LDR when performing non-homogenous assays.
Regardless of one’s assay setup preference INDIGlo LDR delivers rapid lysis of the reporter cells, robust luminescence signal, and sensitive quantification of expressed luciferase activities.
INDIGlo Luciferase Detection Reagent kits provide two reagents in equal volumes:
INDIGlo LDR, formulated as a 2x-concentrated reagent
Dilution Buffer, for optional use if performing non-homogenous assays
[INDIGO Catalog Nos. LDR-10, LDR-25, LDR-50]
WARNING This product is not for human or veterinary use.