A colorimetric plasmin substrate
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D-Val-Leu-Lys-pNA

Item No. 36580

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
D-valyl-L-leucyl-N-(4-nitrophenyl)-L-lysinamide
CAS Number
63589-93-5
Synonyms
  • D-Val-Leu-Lys-p-nitroanilide
  • D-VLK-pNA
  • S 2251
Molecular Formula
C23H38N6O5
Formula Weight
478.6
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Water: Soluble
λmax
221, 315 nm
SMILES
CC(C)[C@@H](N)C(N[C@@H](CC(C)C)C(N[C@@H](CCCCN)C(NC1=CC=C(C=C1)[N+]([O-])=O)=O)=O)=O.Cl.Cl
InChi Code
InChI=1S/C23H38N6O5.2ClH/c1-14(2)13-19(28-23(32)20(25)15(3)4)22(31)27-18(7-5-6-12-24)21(30)26-16-8-10-17(11-9-16)29(33)34;;/h8-11,14-15,18-20H,5-7,12-13,24-25H2,1-4H3,(H,26,30)(H,27,31)(H,28,32);2*1H/t18-,19-,20+;;/m0../s1
InChi Key
VESQMNNSPPEOSZ-ZLARAOTRSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $33

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    5 mg

    $74

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    10 mg

    $130

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    25 mg

    $242

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    Product Description

    D-Val-Leu-Lys-pNA is a colorimetric substrate for plasmin.1,2 Plasmin binds and hydrolyzes D-Val-Leu-Lys-pNA to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of plasmin activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Gohda, K., Fujimori, K., Teno, N., et al. Synthetic substrates specific to activated plasmin can monitor the enzymatic functional status in situ in breast cancer cells. Chem. Biol. Drug Des. 83(1), 52-57 (2014).

    2. Leite, J.A.S., Montoya, C.A., Loveday, S.M., et al. Heat-treatments affect protease activities and peptide profiles of ruminants' milk. Front. Nutr. 8, 626475 (2021).