An endogenous peptide fragment and MRGPRX2 agonist
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PAMP-12 (human, mouse, rat, porcine, bovine) (trifluoroacetate salt)

Item No. 36910

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
L-phenylalanyl-L-arginyl-L-lysyl-L-lysyl-L-tryptophyl-L-asparaginyl-L-lysyl-L-tryptophyl-L-alanyl-L-leucyl-L-seryl-L-argininamide, trifluoroacetate salt
Synonyms
  • PAMP (9-20)
  • Proadrenomedullin N-terminal 12 Peptide
Molecular Formula
C77H119N25O14 • XCF3COOH
Formula Weight
1618.9
Purity
≥95%
Formulation (Request formulation change)
A solid
Peptide Sequence
FRKKWNKWALSR-NH2
Solubility(Learn about Variance in Solubility)
DMF: 50 mg/mlDMSO: 10 mg/mlPBS (pH 7.2): 10 mg/ml
SMILES
N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCCN)C(N[C@@H](CCCCN)C(N[C@H](C(N[C@@H](CC(N)=O)C(N[C@@H](CCCCN)C(N[C@H](C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](CO)C(N[C@H](C(N)=O)CCCNC(N)=N)=O)=O)=O)=O)CC2=CNC3=CC=CC=C23)=O)=O)=O)CC4=CNC5=CC=CC=C45)=O)=O)=O)=O.OC(C(F)(F)F)=O
InChi Code
InChI=1S/C77H119N25O14.C2HF3O2/c1-43(2)35-58(72(113)102-62(42-103)75(116)93-53(64(83)105)28-17-33-88-76(84)85)98-65(106)44(3)92-71(112)59(37-46-40-90-51-23-9-7-21-48(46)51)99-70(111)56(27-13-16-32-80)97-74(115)61(39-63(82)104)101-73(114)60(38-47-41-91-52-24-10-8-22-49(47)52)100-69(110)55(26-12-15-31-79)96-67(108)54(25-11-14-30-78)95-68(109)57(29-18-34-89-77(86)87)94-66(107)50(81)36-45-19-5-4-6-20-45;3-2(4,5)1(6)7/h4-10,19-24,40-41,43-44,50,53-62,90-91,103H,11-18,25-39,42,78-81H2,1-3H3,(H2,82,104)(H2,83,105)(H,92,112)(H,93,116)(H,94,107)(H,95,109)(H,96,108)(H,97,115)(H,98,106)(H,99,111)(H,100,110)(H,101,114)(H,102,113)(H4,84,85,88)(H4,86,87,89);(H,6,7)/t44-,50-,53-,54-,55-,56-,57-,58-,59-,60-,61-,62-;/m0./s1
InChi Key
VZHUBJMTILFZGZ-DRWCHAHRSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

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    10 mg

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    25 mg

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    Product Description

    Proadrenomedullin N-terminal 12 peptide (PAMP-12) is an endogenous peptide fragment found in the adrenal medulla that corresponds to amino acids 9-20 of human PAMP-20 and is involved in hypotension.1,2,3 It is an agonist of MAS-related G protein-coupled receptor family member X2 (MRGPRX2).2 PAMP-12 inhibits forskolin-induced cAMP accumulation in CHO cells expressing human MRGPRX2 (EC50 = 57.2 nM). It selectively induces calcium mobilization in CHO cells expressing MRGPRX2 (EC50 = 41 nM) over those expressing MRGPRX1, MRGPRX3, or MRGPRX4 at 1 µM. PAMP-12 is an antagonist of nicotinic acetylcholine receptors (nAChRs) that inhibits carbachol-induced catecholamine release (IC50 = 1.3 µM) and calcium and sodium influx (IC50s = 0.39 and 0.87 µM, respectively), but not histamine-induced catecholamine release or calcium and sodium influx (IC50s = >1 µM for all), in primary bovine adrenal chromaffin cells.3 It reduces mean arterial blood pressure in normotensive rats when administered at doses ranging from 10 to 50 nmol/kg.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kuwasako, K., Kitamura, K., Ishiyama, Y., et al. Purification and characterization of PAMP-12 (PAMP[9-20]) in porcine adrenal medulla as a major endogenous biologically active peptide. FEBS Lett. 414(1), 108-110 (1997).

    2. Kamohara, M., Matsuo, A., Takasaki, J., et al. Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides. Biochem. Bioph. Res. Commun. 330(4), 1146-1152 (2005).

    3. Kobayashi, H., Yamamoto, R., Kitamura, K., et al. Selective inhibition of nicotinic cholinergic receptors by proadrenomedullin N-terminal 12 peptide in bovine adrenal chromaffin cells. Brain Res. Mol. Brain Res. 87(2), 175-183 (2001).