Host: HEK293 • AA: 22-220 • Tag: C-terminal human IgG1 Fc • MW: 49.5 kDa
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Layilin Isoform 2 Extracellular Domain (human, recombinant)

Item No. 37021

Product Insert (PDF)
Technical Information
Synonyms
  • LAYN
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg determined by the LAL endotoxin assay
Source
Recombinant C-terminal human IgG1 Fc-tagged layilin isoform 2 extracellular domain expressed in HEK293 cells
Amino Acids
22-220
MW
49.5 kDa
Formulation (Request formulation change)
Lyophilized from sterile 50 mM Tris, pH 7.5, with 100 mM glycine and 10 mM sodium chloride
UniProt Accession №
Q6UX15
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $383

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    Layilin is a C-type lectin receptor.1 It is composed of an extracellular region that contains the C-type lectin homology domain, a transmembrane domain, and a cytoplasmic domain. Layilin is ubiquitously expressed and localizes to the cell surface, but has also been found in mitochondria.1,2 It is involved in cell migration, the epithelial-to-mesenchymal transition (EMT), and antitumor immunity and binds to the extracellular matrix proteins hyaluronan and glycosylated collagen, as well as the cytoskeleton-membrane linker proteins talin, merlin, and radixin.1,3,4,5 The layilin isoform 2 extracellular domain binds to melanoma cells in a calcium-dependent manner.5 Knockout of LAYN prevents the accumulation of lymph node-derived CD8+ tumor-infiltrating lymphocytes (TILs) in a competitive adoptive transfer murine colon adenocarcinoma model.6 Layilin protein levels are increased in CD8+ TILs that highly express programmed cell death protein 1 (PD-1) and cytotoxic T lymphocyte protein 4 (CTLA-4) isolated from patients with metastatic melanoma. Cayman’s Layilin Isoform 2 Extracellular Domain (human, recombinant) protein consists of 437 amino acids, has a calculated molecular weight of 49.5 kDa, and a predicted N-terminus of Ala22 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 60-65 kDa due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Borowsky, M.L., and Hynes, R.O. Layilin, a novel talin-binding transmembrane protein homologous with C-type lectins, is localized in membrane ruffles. J. Cell Biol. 143(2), 429-442 (1998).

    2. Tsutiya, A., Arito, M., Tagashira, T., et al. Layilin promotes mitochondrial fission by cyclin-dependent kinase 1 and dynamin-related protein 1 activation in HEK293T cells. Biochem. Biophys. Res. Commun. 549, 143-149 (2021).

    3. Adachi, T., Arito, M., Suematsu, N., et al. Roles of layilin in TNF-α-induced epithelial-mesenchymal transformation of renal tubular epithelial cells. Biochem. Biophys. Res. Commun. 467(1), 63-69 (2015).

    4. Carvalho, A.M., Reis, R.L., and Pashkuleva, I. Hyaluronan receptors as mediators and modulators of the tumor microenvironment. Adv. Healthc. Mater. e2202118 (2022).

    5. Glasgow, J.E., Byrnes, J.R., Barbee, S.D., et al. Identifying and antagonizing the interactions between layilin and glycosylated collagens. Cell Chem. Biol. 29(4), 597-604 (2022).

    6. Mahuron, K.M., Moreau, J.M., Glasgow, J.E., et al. Layilin augments integrin activation to promote antitumor immunity. J. Exp. Med. 217(9), e20192080 (2020).