For immunochemical detection of sPLA2 (Type IB)
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sPLA2 (Type IB) Rabbit Monoclonal Antibody (Clone 001)

Item No. 37088

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Pancreatic Phospholipase A2
  • Pancreatic sPLA2
  • Phospholipase A2 Group IB
  • Phospholipase A2 Group 1B
  • pPLA2
  • sPLA2-IB
  • PLA2G1B
Immunogen
Recombinant mouse sPLA2 (Type IB)
Clone Designation
001
Formulation (Request formulation change)
50 or 100 µl, or 1 ml of protein A-affinity purified monoclonal antibody
Storage Buffer
0.2 μm filtered solution in PBS
Host
Rabbit
Isotype
IgG
Applications
ELISA
Cross Reactivity
((+)) sPLA2 (Type IB)
Species Reactivity
((+)) Mouse((-)) Human
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µl

    $145

    Add

    100 µl

    $246

    Add

    1 ml

    $1,440

    Add
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    Product Description

    Secretory phospholipase A2 (sPLA2) (Type IB) is a calcium-dependent PLA2 superfamily member that is encoded by PLA2G1B in humans.1 It is composed of an N-terminal signal peptide, a highly-conserved calcium-binding loop, a catalytic domain, an sPLA2 Type I-specific disulfide bond that provides stability, and a pancreatic loop. It is primarily expressed in the pancreas but is also expressed in the lung.2 Upon activation, sPLA2 (Type IB) is released into the extracellular space and preferentially hydrolyzes phosphatidylglycerol (PG), phosphatidylcholine (PC), and phosphatidic acid (PA), over phosphatidylserine (PS), phosphatidylethanolamine (PE), and phosphatidylinositol (PI).3 It is involved in lipid nutrient digestion and absorption and also has anthelmintic activity.4 Pla2g1b-/- mice fed a high-fat diet are resistant to obesity and have decreased blood glucose and serum insulin levels and increased fecal lipid levels.5 Mutations in PLA2G1B are associated with central adiposity and colorectal cancer.4 Cayman’s sPLA2 (Type IB) Rabbit Monoclonal Antibody (Clone 001) can be used for ELISA.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Murakami, M., Taketomi, Y., Sato, H., et al. Secreted phospholipase A2 revisited. J. Biochem. 150(3), 233-255 (2011).

    2. Richmond, B.L., and Hui, D.Y. Molecular structure and tissue-specific expression of the mouse pancreatic phospholipase A2 gene. Gene 244(1-2), 65-72 (2000).

    3. Singer, A.G., Ghomashchi, F., Le Calvez, C., et al. Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2. The Journal of Biological Chemisty 277(50), 48535-48549 (2002).

    4. Hui, D.Y. Group 1B phospholipase A2 in metabolic and inflammatory disease modulation. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1864(6), 784-788 (2019).

    5. Huggins, K.W., Boileau, A.C., and Hui, D.Y. Protection against diet-induced obesity and obesity- related insulin resistance in Group 1B PLA2-deficient mice. Am. J. Physiol. Endocrinol. Metab. 283(5), E994-E1001 (2002).