Human Activator Protein-1 Reporter Assay System

This Activator Protein-1 (AP-1) assay kit is an all-inclusive firefly luciferase reporter assay system. In addition to AP-1 Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting test samples, the reference AP-1 activator Phorbol 12-myristate 13-acetate (PMA), Luciferase Detection Reagent, and a cell culture-ready assay plate, and a detailed protocol.

INDIGO's Reporter Cells contain an engineered luciferase reporter gene functionally linked to tandem TPA response element (TRE) sequences positioned immediately upstream of a minimal promoter. Activated AP-1 binds to its TRE to drive Luc gene expression. Thus, quantifying changes in luciferase activity in the treated reporter cells provides a sensitive surrogate measure of changes in AP-1 activity. Accordingly, the principal application of this reporter assay is in the screening of test compounds to quantify any functional activities, either activating or inhibitory, that they may exert against AP-1.

Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryopreservation method yields high cell viability post-thaw and provides the convenience of immediately dispensing healthy reporter cells into assay plates. There is no need for intermediate preparatory steps such as the spin-and-rinse of cells, viability determinations, or cell titer adjustments prior to assay setup.

INDIGO’s Reporter Assays are all-inclusive cell-based assay systems. In addition to AP-1 Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting test samples, the reference AP-1 activator Phorbol 12-myristate 13-acetate (PMA), Luciferase Detection Reagent, and a cell culture-ready assay plate. [INDIGO Catalog Nos. IB2400, IB24001-32, IB24001]

WARNING This product is not for human or veterinary use.