A fluorogenic substrate for caspase-2
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Ac-VDVAD-AFC (trifluoroacetate salt)

Item No. 37351

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-acetyl-L-valyl-L-α-aspartyl-L-valyl-L-alanyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine, trifluoroacetate salt
Synonyms
  • N-Acetyl-Val-Asp-Val-Ala-Asp-AFC
  • N-Acetyl-Val-Asp-Val-Ala-Asp-7-amino-4-Trifluoromethylcoumarin
  • Caspase-2 Substrate (Fluorogenic)
Molecular Formula
C33H41F3N6O12 • XCF3COOH
Formula Weight
770.7
Purity
≥95%
Emission
505 nm
Excitation
400 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Water: Soluble
SMILES
OC(C[C@H](NC([C@H](C)NC([C@@H](NC([C@H](CC(O)=O)NC([C@@H](NC(C)=O)C(C)C)=O)=O)C(C)C)=O)=O)C(NC(C=C1O2)=CC=C1C(C(F)(F)F)=CC2=O)=O)=O.OC(C(F)(F)F)=O
InChi Code
InChI=1S/C33H41F3N6O12.C2HF3O2/c1-13(2)26(38-16(6)43)32(53)41-21(12-24(46)47)30(51)42-27(14(3)4)31(52)37-15(5)28(49)40-20(11-23(44)45)29(50)39-17-7-8-18-19(33(34,35)36)10-25(48)54-22(18)9-17;3-2(4,5)1(6)7/h7-10,13-15,20-21,26-27H,11-12H2,1-6H3,(H,37,52)(H,38,43)(H,39,50)(H,40,49)(H,41,53)(H,42,51)(H,44,45)(H,46,47);(H,6,7)/t15-,20-,21-,26-,27-;/m0./s1
InChi Key
PNDAWJNREIOBPU-IQYSWUAISA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    500 µg

    $57

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    1 mg

    $107

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    5 mg

    $307

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    10 mg

    $556

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    Product Description

    Ac-VDVAD-AFC is a fluorogenic substrate for caspase-2.1,2 Upon enzymatic cleavage by caspase-2, 7-amino-4-trifluoromethylcoumarin (AFC) is released and its fluorescence can be used to quantify caspase-2 activity. AFC displays excitation/emission maxima of 400/505 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Moriya, R., Uehara, T., and Nomura, Y. Mechanism of nitric oxide-induced apoptosis in human neuroblastoma SH-SY5Y cells. FEBS Lett. 484(3), 253-260 (2000).

    2. Talanian, R.V., Quinlan, C., Trautz, S., et al. Substrate specificities of caspase family proteases. The Journal of Biological Chemisty 272(15), 9677-9682 (1997).