An Hsf1 inhibitor
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

DTHIB

Item No. 37433

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-(4-chloro-3-nitrophenyl)-N′-(4-fluorophenyl)-urea
CAS Number
897326-30-6
Synonyms
  • Direct Targeted Heat Shock Factor 1 Inhibitor
  • Direct Targeted Hsf1 Inhibitor
Molecular Formula
C13H9ClFN3O3
Formula Weight
309.7
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: SolubleMethanol: Soluble
λmax
256 nm
SMILES
O=C(NC1=CC=C(C([N+]([O-])=O)=C1)Cl)NC2=CC=C(C=C2)F
InChi Code
InChI=1S/C13H9ClFN3O3/c14-11-6-5-10(7-12(11)18(20)21)17-13(19)16-9-3-1-8(15)2-4-9/h1-7H,(H2,16,17,19)
InChi Key
DWVIOMKFHSRQQM-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    25 mg

    $169

    Add

    50 mg

    $303

    Add

    100 mg

    $471

    Add

    250 mg

    $842

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    DTHIB is an inhibitor of heat shock factor 1 (Hsf1).1 It binds to the DNA-binding domain of Hsf1 (Kd = 160 nM) and inhibits heat-induced increases in the Hsf1 target proteins heat shock protein 27 (Hsp27) and Hsp70 levels in mouse embryonic fibroblasts (MEFs) when used at a concentration of 10 µM. It decreases nuclear but not cytoplasmic levels of Hsf1 in C4-2 prostate cancer cells, an effect that can be blocked by the proteasome inhibitor MG132. DTHIB decreases the viability of 22Rv1, C4-2, and PC3 prostate cancer cells (EC50 = 1.6, 1.2, and 3 µM, respectively) and induces cell cycle arrest at the G1 phase in C4-2 cells. In vivo, DTHIB (5 mg/kg) induces tumor regression in a TRAMP-C2 murine prostate cancer model.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dong, B., Jaeger, A.M., Hughes, P.F., et al. Targeting therapy-resistant prostate cancer via a direct inhibitor of the human heat shock transcription factor 1. Sci. Transl. Med. 12(574), eabb5647 (2020).