An inhibitor of LIF activity
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EC330

Item No. 37551

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(11β,17β)-11-(4-cyclopropylphenyl)-17-(1,1-difluoro-2-propyn-1-yl)-17-hydroxy-estra-4,9-dien-3-one
CAS Number
2016795-77-8
Molecular Formula
C30H32F2O2
Formula Weight
462.6
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlDMSO: 10 mg/mlEthanol: 20 mg/ml
SMILES
C[C@@]12[C@](CC[C@@]2(O)C(F)(F)C#C)([H])[C@@]3([H])C([C@@H](C4=CC=C(C5CC5)C=C4)C1)=C6C(CC3)=CC(CC6)=O
InChi Code
InChI=1S/C30H32F2O2/c1-3-30(31,32)29(34)15-14-26-24-12-10-21-16-22(33)11-13-23(21)27(24)25(17-28(26,29)2)20-8-6-19(7-9-20)18-4-5-18/h1,6-9,16,18,24-26,34H,4-5,10-15,17H2,2H3/t24-,25+,26-,28-,29-/m0/s1
InChi Key
OWPPSPZDYGHPAN-GCNJZUOMSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $109

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    5 mg

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    10 mg

    $818

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    Cayman Chemical
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    Product Description

    EC330 is an inhibitor of leukemia inhibitory factor (LIF) activity.1 It binds to the LIF receptor (LIFR) and is selectively cytotoxic to MCF-7 cells ectopically overexpressing LIF (IC50 = 0.075 µM) over non-LIF-overexpressing MCF-7 cells (IC50 = 0.245 µM). EC330 reduces ectopic LIF expression-induced increases in the migration of MCF-7 and MDA-MB-231 cells when used at concentrations of 5 and 15 nM, respectively. It also induces ferroptosis in 293T and 786-O cells and reduces the levels of glutathione (GSH) and the intracellular activity of glutathione peroxidase (GPX) in vitro.2 EC330 (1 mg/kg) reduces tumor burden to a greater extent in a mouse xenograft model using MDA-MB-231 cells with ectopic LIF expression than when using MDA-MB-231 cells without ectopic LIF expression.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Yue, X., Wu, F., Wang, J., et al. EC330, a small-molecule compound, is a potential novel inhibitor of LIF signaling. J. Mol. Cell Biol. 12(6), 477-480 (2020).

    2. Feng, C.-Z., Li, N.-Z., Hu, X.-B., et al. The LIFR-targeting small molecules EC330/EC359 are potent ferroptosis inducers. Genes Dis. 10(3), 735-738 (2022).