Citrullinated Nucleophosmin (human, recombinant)

Nucleophosmin is a nucleolar phosphoprotein that is involved in diverse biological processes, including ribosome maturation, DNA repair, and mitotic spindle assembly.¹ It is highly post-translationally modified and composed of an oligomerization domain, which contains two nuclear export signals, three acidic domains, an intrinsically disordered region, which contains two nuclear localization signals, and a C-terminal RNA-binding domain.^1,2 Nucleophosmin is ubiquitously expressed and shuttles between the nucleolus and cytoplasm; however, alternative splicing of NPM1 produces a variant that lacks the RNA-binding domain and nucleolar localization signal and is instead localized throughout the nucleus.^3,1,2 Nucleophosmin has histone- and protein chaperone activity and plays a role in ribosome assembly and export, centrosome duplication, cell cycle control, the stress response, and embryogenesis.¹ Nucleophosmin citrullinated by PAD4 increases p53 stability and radiation sensitivity in a thyroid cancer cell line exposed to radiation.⁴ This product contains purified nucleophosmin (Item No. 37621) that has been modified with PAD4, which is subsequently depleted by affinity chromatography.

WARNING This product is not for human or veterinary use.

1. Cela, I., Di Matteo, A., and Federici, L. Nucleophosmin in Its interaction with ligands. Int. J. Mol. Sci. 21(14), 4885 (2020).

2. Okuwaki, M. The structure and functions of NPM1/Nucleophsmin/B23, a multifunctional nucleolar acidic protein. J. Biochem. 143(4), 441-448 (2008).

3. Cordell, J.L., Pulford, K.A., Bigerna, B., et al. Detection of normal and chimeric nucleophosmin in human cells. Blood 93(2), 632-642 (1999).

4. Na, J., Lee, C.-H., Chung, J.-K., et al. Overexpression of both human sodium Iodide symporter (NIS) and BRG1-bromodomain synergistically enhances radioiodine sensitivity by Sstabilizing p53 through NPM1 expression. Int. J. Mol. Sci. 24(3), 2761 (2023).