A fluorescent probe for sulfane sulfur species
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Sulfane Sulfur Probe 4

Item No. 37921

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-mercapto-benzoic acid, 6′-[(2-mercaptobenzoyl)oxy]-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3′-yl ester
CAS Number
1810731-98-6
Synonyms
  • SSP4
Molecular Formula
C34H20O7S2
Formula Weight
604.6
Purity
≥95%
Emission
515 nm
Excitation
494 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Chloroform: SolubleDMF: SolubleDMSO: Soluble
λmax
227 nm
SMILES
O=C(O1)C2=C(C=CC=C2)C31C4=CC=C(OC(C5=C(S)C=CC=C5)=O)C=C4OC6=C3C=CC(OC(C7=CC=CC=C7S)=O)=C6
InChi Code
InChI=1S/C34H20O7S2/c35-31(22-8-2-5-11-29(22)42)38-19-13-15-25-27(17-19)40-28-18-20(39-32(36)23-9-3-6-12-30(23)43)14-16-26(28)34(25)24-10-4-1-7-21(24)33(37)41-34/h1-18,42-43H
InChi Key
QIJWBZMASQXNLS-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 mg

    $349

    Add
    Product Description

    Sulfane sulfur probe 4 (SSP4) is a fluorescent probe for sulfane sulfur species, including endogenously generated hydrogen sulfide-derived polysulfides, hydropersulfides, and persulfides.1 In the presence of sulfane sulfur species, SSP4 is subject to a nucleophilic reaction resulting in the formation of fluorescein, which displays excitation/emission maxima of 494/515 nm, respectively, and two molecules of 1,2-benzodithiol-3-one.1,2 SSP4 has been used to determine localization of H2S3 in primary mouse neurons and to detect polysulfides in L. japonicus seedling roots.3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bibli, S.-I., Luck, B., Zukunft, S., et al. A selective and sensitive method for quantification of endogenous polysulfide production in biological samples. Redox Biol. 18, 295-304 (2018).

    2. Johnson, I., and Spence, M.T.Z. The molecular probes handbook—a guide to fluorescent probes and labeling technologies. (2010).

    3. Kimura, Y., Toyofuku, Y., Koike, S., et al. Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain. Sci. Rep. 5:14774, (2015).

    4. Fukudome, M., Shimada, H., Uchi, N., et al. Reactive sulfur species interact with other signal molecules in root nodule symbiosis in Lotus japonicus. Antioxidants (Basel) 9(2), 145 (2020).